Pinto Camila Moreira, Schnepper Amanda Piveta, Trindade Pedro Henrique Esteves, Cardoso Luiz Henrique, Fioretto Matheus Naia, Justulin Luís Antônio, Zanelli Cleslei Fernando, Valente Guilherme Targino
Laboratory of Applied Biotechnology. São Paulo State University (UNESP). Botucatu, Brazil.
Department of Population Health and Pathobiology College of Veterinary Medicine, North Carolina State University (NCSU) Raleigh, USA.
Heliyon. 2024 May 19;10(10):e31561. doi: 10.1016/j.heliyon.2024.e31561. eCollection 2024 May 30.
Elevated ethanol concentrations in yeast affect the plasma membrane. The plasma membrane in yeast has many lipid-protein complexes, such as Pma1 (MCP), Can1 (MCC), and the eisosome complex. We investigated the response of eisosomes, MCPs, and membraneless structures to ethanol stress. We found a correlation between ethanol stress and proton flux with quick acidification of the medium. Moreover, ethanol stress influences the symporter expression in stressed cells. We also suggest that acute stress from ethanol leads to increases in eisosome size and SG number: we hypothesized that eisosomes may protect APC symporters and accumulate an mRNA decay protein in ethanol-stressed cells. Our findings suggest that the joint action of these factors may provide a protective effect on cells under ethanol stress.
酵母中乙醇浓度升高会影响质膜。酵母中的质膜有许多脂质 - 蛋白质复合物，如Pma1（MCP）、Can1（MCC）和胞膜窖复合体。我们研究了胞膜窖、MCP和无膜结构对乙醇胁迫的反应。我们发现乙醇胁迫与质子通量以及培养基的快速酸化之间存在相关性。此外，乙醇胁迫会影响应激细胞中同向转运体的表达。我们还提出，乙醇引起的急性应激会导致胞膜窖大小和SG数量增加：我们假设胞膜窖可能保护APC同向转运体，并在乙醇应激细胞中积累一种mRNA衰变蛋白。我们的研究结果表明，这些因素的共同作用可能为乙醇胁迫下的细胞提供保护作用。
