一项基于孟德尔随机化方法的奶酪摄入量与龋齿发生关系的研究。

A study on the relationship between cheese intake and caries occurrence based on Mendelian randomization method.

作者信息

Zhang Ruoyan, Jiang Huaxiang, Qin Zishun, Wang Jun, Hu Xi

机构信息

Department of Stomatology, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330000, China.

JXHC Key Laboratory of Periodontology (The Second Affiliated Hospital of Nanchang University), Nanchang, China.

出版信息

Heliyon. 2024 May 11;10(10):e30856. doi: 10.1016/j.heliyon.2024.e30856. eCollection 2024 May 30.

DOI:10.1016/j.heliyon.2024.e30856

PMID:38818144

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11137352/

Abstract

OBJECTIVE

The study aims to investigate the causal relationship between cheese intake and caries occurrence by a two-sample Mendelian randomization method (TSMR).

METHODS

Data from a genome-wide association study (GWAS) on cheese intake as an exposure factor were collected, and dental caries was the outcome variable, appropriate SNPs were selected as instrumental variables (IVs). The TSMR was analyzed by the inverse variance weighting (IVW) method, weighted median method, MR-Egger regression method, simple model and weighted model.

RESULTS

We identified forty-four single-nucleotide polymorphisms (SNPs) in the gene encoding group-specific component (cheese) that were associated with cheese intake, and IVW was adopted. The IVW method supported a relationship between cheese intake and the risk of dental caries occurrence[OR,1.00(95 %CI,0.99-1.00), P = 0.039 < 0.05]. There was no horizontal pleiotropy between the IVs(b = -0.0037, P = 0.39), and the sensitivity analysis using the "leave-one-out" method was robust to causal effects.

CONCLUSION

The results of the TSMR analysis supported that an appropriate intake of cheese could reduce the occurrence of dental caries.

摘要

目的

本研究旨在通过两样本孟德尔随机化方法（TSMR）探讨奶酪摄入量与龋齿发生之间的因果关系。

方法

收集以奶酪摄入量作为暴露因素的全基因组关联研究（GWAS）数据，将龋齿作为结局变量，选择合适的单核苷酸多态性（SNPs）作为工具变量（IVs）。采用逆方差加权法（IVW）、加权中位数法、MR-Egger回归法、简单模型和加权模型对TSMR进行分析。

结果

我们在编码类特异性成分（奶酪）的基因中鉴定出44个与奶酪摄入量相关的单核苷酸多态性（SNPs），并采用了IVW法。IVW法支持奶酪摄入量与龋齿发生风险之间存在关联[比值比，1.00（95%可信区间，0.99 - 1.00），P = 0.039 < 0.05]。IVs之间不存在水平多效性（b = -0.0037，P = 0.39），使用“留一法”的敏感性分析对因果效应具有稳健性。