Revoltella R P, Butler R H, Musiani P, Piantelli M, Ranelletti F O
Scand J Immunol. 1985 Jan;21(1):11-20. doi: 10.1111/j.1365-3083.1985.tb01397.x.
Interleukin-1 (IL-1) production by the human monocyte-like cloned cell line CM-SM has been investigated as a function of the state of cell differentiation. CM-SM cells were induced to differentiate along the monocyte-macrophage lineage by bacterial lipopolysaccharides (LPS) or by 12-O-tetradecanoyl-phorbol-13-acetate (TPA). Cell differentiation was studied by various morphological, functional, cytochemical, and immunological variables, whereas IL-1 activity in the supernatants was measured by the lectin-primed thymocyte proliferation assay. Unstimulated CM-SM cells constitutively produced small amounts of IL-1, and most of the cells appeared relatively undifferentiated. LPS induced cell differentiation, but the effect was reversible, and the cells, in general, did not acquire a capacity for phagocytosis. IL-1 levels were increased about 10-fold over the controls. TPA induced further cell differentiation to macrophage-like cells capable of phagocytosis. IL-1 activity could not be measured directly in the supernatants owing to the synergistic effect of TPA in the assay system. Unequivocal removal of the phorbol was not achieved, but the data indicated that the 'real' levels of IL-1 in the TPA-induced cultures were not significantly higher than those from LPS-induced cultures.
作为细胞分化状态的一个函数，已经对人单核细胞样克隆细胞系CM-SM产生白细胞介素-1（IL-1）的情况进行了研究。通过细菌脂多糖（LPS）或12-O-十四酰佛波醇-13-乙酸酯（TPA）诱导CM-SM细胞沿单核细胞-巨噬细胞谱系分化。通过各种形态学、功能、细胞化学和免疫学变量研究细胞分化，而通过凝集素预处理的胸腺细胞增殖试验测量上清液中的IL-1活性。未刺激的CM-SM细胞组成性地产生少量IL-1，并且大多数细胞看起来相对未分化。LPS诱导细胞分化，但这种效应是可逆的，并且一般来说细胞没有获得吞噬能力。IL-1水平比对照增加了约10倍。TPA诱导细胞进一步分化为能够吞噬的巨噬细胞样细胞。由于TPA在测定系统中的协同作用，无法直接测量上清液中的IL-1活性。未能明确去除佛波醇，但数据表明TPA诱导培养物中IL-1的“实际”水平并不显著高于LPS诱导培养物中的水平。
