Ito S, Suzuki T, Momotsu T, Isemura S, Saitoh E, Sanada K, Shibata A
Acta Endocrinol (Copenh). 1985 Jan;108(1):130-4. doi: 10.1530/acta.0.1080130.
An indirect immunofluorescence technique using antisera against salivary peptide P-C and against salivary Protein C was carried out on the laryngeal, tracheal and bronchial glands to examine whether salivary peptide P-C-like immunoreactivity, recently demonstrated in the serous cells of the human salivary glands, was also present in those of laryngeal, tracheal and bronchial glands and to ascertain whether salivary peptide P-C is a fragment of salivary Protein C or not. Salivary peptide P-C-like immunoreactivity was present in the serous cells of the human laryngeal, tracheal and bronchial glands. Observation of serial sections immunostained with two kinds of antisera revealed that cells reacting with antisera against salivary peptide P-C were identical to those reacting with antisera against salivary Protein C pre-incubated with salivary peptide P-C. The finding implied that salivary peptide P-C and salivary Protein C, originally isolated from human saliva, were also present in the serous cells of tissues other than the salivary glands. Furthermore, analysis of the primary structure of salivary peptide P-C and salivary Protein C together with the present morphological finding suggests that salivary peptide P-C is a COOH-terminal fragment of salivary Protein C. Thus, salivary Protein C and salivary peptide P-C may play some role in the function of the serous cells of the salivary and laryngo-tracheo-bronchial glands.
运用抗唾液肽P-C抗血清和抗唾液蛋白C抗血清的间接免疫荧光技术，对喉、气管和支气管腺体进行检测，以探究近期在人类唾液腺浆液细胞中发现的唾液肽P-C样免疫反应性是否也存在于喉、气管和支气管腺体的浆液细胞中，并确定唾液肽P-C是否为唾液蛋白C的片段。人类喉、气管和支气管腺体的浆液细胞中存在唾液肽P-C样免疫反应性。用两种抗血清对连续切片进行免疫染色观察发现，与抗唾液肽P-C抗血清反应的细胞与与用唾液肽P-C预孵育的抗唾液蛋白C抗血清反应的细胞相同。这一发现表明，最初从人唾液中分离出的唾液肽P-C和唾液蛋白C也存在于唾液腺以外组织的浆液细胞中。此外，对唾液肽P-C和唾液蛋白C一级结构的分析以及目前的形态学发现表明，唾液肽P-C是唾液蛋白C的COOH末端片段。因此，唾液蛋白C和唾液肽P-C可能在唾液腺以及喉-气管-支气管腺体浆液细胞的功能中发挥某些作用。
