Ito S, Suzuki T, Momotsu T, Tsuda A, Isemura S, Saitoh E, Sanada K, Shibata A
Nihon Naibunpi Gakkai Zasshi. 1984 Sep 20;60(9):1080-90. doi: 10.1507/endocrine1927.60.9_1080.
In order to clarify the intracellular localization of salivary peptide P-C like immunoreactivity in the human pancreatic B-cells, an immuno-electronmicroscopical study using protein A-gold technique was carried out on the human foetal pancreas. Salivary peptide P-C like immunoreactivity was present in some of insulin secretory granules while insulin like immunoreactivity was found in all insulin secretory granules. The finding suggested that new substance in addition to insulin and its precursor was present in the insulin secretory granules of the human pancreatic B-cells. Furthermore, the finding seemed to explain the previous study that development of salivary peptide'P-C like immunoreactivity in the foetal pancreatic B-cells was immature in compared to that in the human adult pancreatic B-cells. In addition, to examine whether or not, salivary peptide P-C like immunoreactivity in the human pancreatic B-cells is Salivary Protein C, an indirect immunofluorescence technique using three kinds of antisera against salivary peptide P-C, Salivary Protein C and salivary peptide P-B, and using their antigens was undertaken on the adult pancrease. From the results of the study, it was thought that salivary peptide P-C like immunoreactivity in the human pancreas was neither Salivary Protein C itself, nor salivary peptide P-B itself, but was either salivary peptide P-C itself or unknown substance which had the common antigenic determinant with salivary peptide P-C, P-B and Salivary Protein C, since salivary peptide P-C like immunoreactivity in the human pancreas disappeared in the sections in which P-C antisera preincubated with each of salivary peptide P-C, P-B and Salivary Protein C were used as the primary antisera. Thus, although new substance was present in the insulin secretory granules of the human pancreatic B-cells, its pathophysiological role remained to be elucidated.
为了阐明人胰腺β细胞中唾液肽P-C样免疫反应性的细胞内定位，利用蛋白A-金技术对人胎儿胰腺进行了免疫电子显微镜研究。在一些胰岛素分泌颗粒中存在唾液肽P-C样免疫反应性，而在所有胰岛素分泌颗粒中均发现胰岛素样免疫反应性。这一发现表明，人胰腺β细胞的胰岛素分泌颗粒中除胰岛素及其前体之外还存在新物质。此外，这一发现似乎可以解释之前的研究，即胎儿胰腺β细胞中唾液肽P-C样免疫反应性的发育与成人胰腺β细胞相比是不成熟的。另外，为了检测人胰腺β细胞中的唾液肽P-C样免疫反应性是否为唾液蛋白C，利用针对唾液肽P-C、唾液蛋白C和唾液肽P-B的三种抗血清及其抗原，对成人胰腺进行了间接免疫荧光技术检测。从研究结果来看，认为人胰腺中的唾液肽P-C样免疫反应性既不是唾液蛋白C本身，也不是唾液肽P-B本身，而是唾液肽P-C本身或与唾液肽P-C、P-B和唾液蛋白C具有共同抗原决定簇的未知物质，因为在将与唾液肽P-C、P-B和唾液蛋白C预温育的P-C抗血清用作一抗的切片中，人胰腺中的唾液肽P-C样免疫反应性消失了。因此，尽管人胰腺β细胞的胰岛素分泌颗粒中存在新物质，但其病理生理作用仍有待阐明。
