Bass I A, Danilevskaia O N, Mekhedov S L, Gorlenko Zh M, Fedoseeva V B
Mol Biol (Mosk). 1979 Sep-Oct;13(5):1052-63.
We studied the rate of synthesis of beta- and beta'-subunits of DNA-dependent RNA polymerase and the rate of beta-polypeptide mRNA synthesis in rifampicin-treated bacteria. The antibiotic doses used did not significantly inhibit the total RNA and protein synthesis in rifampicin-sensitive bacteria. For RNA-DNA hybridization experiments a pOD162 plasmid was constructed carrying a fragment of the rpoB gene and no other chromosome DNA regions. It is found that low doses of rifampicin cause an absolute and differential increase in the rate of synthesis of the specific mRNA for the beta-subunit, suggesting a stimulation of the corresponding gene transcription. However the absolute transcription stimulation does not fully correlate with the relative acceleration of beta-mRNA and the corresponding polypeptide synthesis. The stimulating effect of rifampicin on the beta-polypeptide synthesis was demonstrated also in a coupled system of transcription and translation directed by lambda rifd 47 DNA. The possible mechanisms of the rifampicin action are discussed.
我们研究了利福平处理的细菌中依赖DNA的RNA聚合酶β和β'亚基的合成速率以及β多肽mRNA的合成速率。所用抗生素剂量并未显著抑制利福平敏感细菌中的总RNA和蛋白质合成。为进行RNA-DNA杂交实验，构建了携带rpoB基因片段且无其他染色体DNA区域的pOD162质粒。发现低剂量利福平会使β亚基特异性mRNA的合成速率出现绝对且有差异的增加，表明相应基因转录受到刺激。然而，绝对转录刺激与β-mRNA和相应多肽合成的相对加速并不完全相关。利福平对β多肽合成的刺激作用在由λrifd 47 DNA指导的转录和翻译偶联系统中也得到了证实。文中讨论了利福平作用的可能机制。
