Lideman L F, Ponomarenko O I, Shakulov R S
Mol Biol (Mosk). 1981 Mar-Apr;15(2):316-22.
When E. coli protein synthesis was blocked by chloramphenicol (100 micrograms/ml) or by essential amino acid deprivation, the transcription rates of rplKAJL genes (the ones for L11, L4, L10 and L7/L12 ribosomal proteins) and adjacent rpoBC genes (genes for RNA polymerase beta- and beta'-polypeptides) have been non-coordinately changed. The level of the gene transcription rate was obtained from RNA--DNA hybridization assays with E. coli pulse-labelled RNA and pJC703 or pJC720 plasmid DNA. The transcription of ribosomal protein genes has been found to be uncoupled with translation and controlled by the allelic state of relA gene. Conversely, the effective transcription of proBC gene was relA independent and coupled with translation of the mRNA. Chloramphenicol-induced transcription polarity within rplKAJL-rpoBC chromosome region can be suppressed by 10 micrograms/ml rifampicin.
当大肠杆菌的蛋白质合成被氯霉素（100微克/毫升）或必需氨基酸剥夺所阻断时，rplKAJL基因（编码L11、L4、L10和L7/L12核糖体蛋白的基因）和相邻的rpoBC基因（编码RNA聚合酶β和β'多肽的基因）的转录速率发生了不协调的变化。基因转录速率水平是通过用大肠杆菌脉冲标记的RNA与pJC703或pJC720质粒DNA进行RNA-DNA杂交试验获得的。已发现核糖体蛋白基因的转录与翻译解偶联，并受relA基因的等位基因状态控制。相反，proBC基因的有效转录不依赖relA，且与mRNA的翻译偶联。氯霉素诱导的rplKAJL-rpoBC染色体区域内的转录极性可被10微克/毫升的利福平抑制。
