Guangdong Pharmaceutical University, 280 Outer Ring East Road, University City, Guangzhou, Guangdong Province, 510006, China.
Sinopharm Group Medi-World Pharmaceutical Co. Ltd, No. 2, Keyuan Hengsan Road, High tech Park, Ronggui Street, Shunde District, Foshan, Guangdong Province, 528303, China.
J Chromatogr Sci. 2024 Aug 20;62(7):635-648. doi: 10.1093/chromsci/bmae034.
Danggui-Jianzhong decoction (DGJZ) is a famous classical traditional Chinese medicine formula, which ingredients are complex and the quality is difficult to control. Our study aimed to identify the overall chemical profile of DGJZ qualitatively by ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and UPLC. A total of 77 components, including terpenoids, flavonoids, phenolic acids, gingerols and other components, were firstly detected and characterized by UPLC-Q-TOF-MS and 18 peaks marked after analyzing the UPLC fingerprint. Finally, paeoniflorin, liquiritin, ferulic acid, cinnamic acid, glycyrrhizic acid and 6-gingerol were quantified, which was validated in terms of linearity, precision, accuracy, repeatability and recovery. Taken together, the chemical constitutes of DGJZ were systematically identified and a reliable quantitative method coupled with fingerprint analysis was successfully employed for evaluating the holistic quality, which will provide a robust foundation for the quality control of DGJZ.
当归建中汤（DGJZ）是一种著名的经典中药方剂，其成分复杂，质量难以控制。本研究旨在通过超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF-MS）和 UPLC 定性地分析 DGJZ 的整体化学特征。通过 UPLC-Q-TOF-MS 检测并鉴定了 77 种成分，包括萜类、黄酮类、酚酸类、姜辣素等成分，通过分析 UPLC 指纹图谱标记了 18 个峰。最后，对芍药苷、甘草苷、阿魏酸、肉桂酸、甘草酸和 6-姜辣素进行了定量分析，并从线性、精密度、准确度、重复性和回收率等方面进行了验证。综上所述，本研究系统地鉴定了 DGJZ 的化学成分，并成功地采用了一种可靠的定量方法结合指纹图谱分析来评估整体质量，这将为 DGJZ 的质量控制提供坚实的基础。
