School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China.
Nat Prod Res. 2021 Apr;35(7):1207-1211. doi: 10.1080/14786419.2019.1641808. Epub 2019 Jul 17.
Xiaoyaosan is one of the famous formulas treating for liver, spleen and blood deficiency syndrome along Chinese history. Their five main components, paeoniflorin, ferulic acid, glycyrrhizic acid, liquiritin, and atractylenolide I are believed to partly representative of this formula. Ultra performance liquid chromatography method was newly established, UPLC BEH-C18 column (2.1 × 100 mm, 1.7 μm) with acetonitrile - 0.1% phosphate acid gradient elution system, in 0.4 mL/min and the temperature was 30 °C; Detection wavelengths were optimized separately. The five components were linear within their linear range ( ≥ 0.9991), average recovery 97.63%∼102.83%, RSD 1.88%∼4.38%. The newly established method is accurate, rapid, and convenient, with satisfied separation performance to quantify multi-components in formula and preparations of Xiaoyaosan. It will provide a reliable reference for the quality evaluation of both Xiaoyaosan formula and preparations during the process of manufacture, administration and clinical application in the future.
逍遥散是中医历史上治疗肝脾血虚证的著名方剂之一。其五种主要成分白芍苷、阿魏酸、甘草酸、甘草苷和白术内酯 I 被认为是该方剂的部分代表性成分。本文建立了一种新的超高效液相色谱法，采用 UPLC BEH-C18 柱（2.1×100mm，1.7μm），以乙腈-0.1%磷酸梯度洗脱系统，流速为 0.4mL/min，柱温为 30°C；分别优化检测波长。五种成分在其线性范围内（≥0.9991）呈线性，平均回收率为 97.63%∼102.83%，RSD 为 1.88%∼4.38%。该方法准确、快速、方便，具有满意的分离性能，可用于定量测定逍遥散配方及制剂中的多种成分。为今后逍遥散配方及制剂在生产、管理和临床应用过程中的质量评价提供了可靠的参考。
