Fung J C, Clogston A, Swenson P, Kaplan M
Am J Clin Pathol. 1985 Feb;83(2):196-9. doi: 10.1093/ajcp/83.2.196.
Commercially available kits were used for the detection of Toxoplasma-specific IgM antibodies. False positive IgM results were observed in whole sera containing Toxoplasma-specific antibodies together with rheumatoid factor when tested by immunofluorescence (IFA). False negative IgM results occurred in whole sera containing competing levels of Toxoplasma-specific IgG antibodies, as indicated by the IFA:IgM-M ratio. False positive and false negative IgM results often occurred when whole sera were tested. These false reactions were eliminated by fractioning IgM from IgG using the Isolab's IgM Isolation System. All five sera in this study with an IFA titer to Toxoplasma of greater than or equal to 1:16,384 also contained Toxoplasma-specific IgM antibodies. This suggests that sera with high titers to Toxoplasma should be tested for Toxoplasma-specific IgM antibodies.
使用市售试剂盒检测弓形虫特异性IgM抗体。当通过免疫荧光法（IFA）检测时，在含有弓形虫特异性抗体以及类风湿因子的全血清中观察到IgM结果呈假阳性。如IFA:IgM-M比值所示，在含有竞争性水平的弓形虫特异性IgG抗体的全血清中出现IgM结果假阴性。检测全血清时经常出现IgM结果假阳性和假阴性。使用Isolab的IgM分离系统从IgG中分离IgM可消除这些假反应。本研究中所有五份对弓形虫的IFA滴度大于或等于1:16,384的血清也都含有弓形虫特异性IgM抗体。这表明应对弓形虫高滴度血清检测弓形虫特异性IgM抗体。
