[大肠杆菌苯丙氨酰 - tRNA合成酶与tRNA Phe复合物的化学修饰]
[Chemical modification of the complex of tRNA Phe with phenylalanyl-tRNA synthetase from Escherichia coli].
作者信息
Ankilova V N, Vlasov V V, Mamaev S V, Nuzhdina N A
出版信息
Mol Biol (Mosk). 1979 Sep-Oct;13(5):994-1000.
PMID:388193
Abstract
Alkylation of E. coli tRNAPhe, bound to the cognate synthetase was investigated. The alkylating reagent is a derivative of 2-chloroethylamine: 2',3'-O-[4(N-2-chloroethyl-N-methylamino)-benzylidene]-uridine-5'-methylphosphate. It was found that the enzyme protects from the reaction D-stem (guanosine G24) and the region of juxtaposition of acceptor stem and D-stem (S4U8 and C13) in the tRNAPhe.
摘要
对与同源合成酶结合的大肠杆菌苯丙氨酸转运核糖核酸（tRNAPhe）的烷基化进行了研究。烷基化试剂是2-氯乙胺的衍生物：2',3'-O-[4（N-2-氯乙基-N-甲基氨基）-亚苄基]-尿苷-5'-甲基磷酸酯。研究发现，该酶可保护tRNAPhe中的D茎（鸟苷G24）以及受体茎与D茎并列的区域（S4U8和C13）免受反应影响。
相似文献
1
[Chemical modification of the complex of tRNA Phe with phenylalanyl-tRNA synthetase from Escherichia coli].
Mol Biol (Mosk). 1979 Sep-Oct;13(5):994-1000.
2
[Alkylation of tRNA-Phe free and bound to phenylalanyl-tRNA synthetase with 4-(N-2-chloroethyl-N-methylamino)benzylamine].
Mol Biol (Mosk). 1981 Jan-Feb;15(1):45-53.
3
4
[Modification of one tRNA recognition site of phenylalanyl-tRNA synthetase from E. coli MRE-600 with N-chlorambucilyl-phenylalanyl-tRNA].
Mol Biol (Mosk). 1978 Sep-Oct;12(5):1085-95.
5
[Modification of the alpha-subunit of phenylalanyl-tRNA synthetase from E. coli MRE-600 with N-chlorambucilyl-phenylalanyl-tRNA].
Biokhimiia. 1979 Mar;44(3):570-2.
6
[Role of the lysine residues in the phenylalanyl-tRNA synthetase substrates interaction].
Mol Biol (Mosk). 1978 Sep-Oct;12(5):1096-104.
7
[Specific modification of phenylalanine:tRNA-ligases of E. coli MRE-600 with N-chlorambucilyl-14c-phenylalanyl-tRNA].
Mol Biol (Mosk). 1975 Jul-Aug;9(4):509-15.
8
[Interaction between tRNA-recognizing sites of phenylalanyl-tRNA synthetase from Escherichia coli MRE-600].
Mol Biol (Mosk). 1982 Sep-Oct;16(5):984-90.
9
[Influence of the modification of Phe-tRNA synthetase from Escherichia coli by lysine- and arginine-specific reagent on the ionic interactions of the enzyme with tRNA Phe].
Mol Biol (Mosk). 1979 Jul-Aug;13(4):788-97.
10
Zotero 插件