Vlasov V V, Lavrik O I, Mamaev S V, Khodyreva S N, Chizhikov V E
Mol Biol (Mosk). 1980 May-Jun;14(3):531-8.
Photoreactive derivatives of tRNAPhe (E. coli) were synthesized by alkylation of the tRNAPhe with 4-(N-2-chloroethyl-N-methylamino)benzylamine and subsequent treatment with 1.4--dinitro-5-fluorophenylazide. The derivatives are active in binding with ribosomes and phenylalanyl-tRNA synthetase when the extent of modification is lower than 3 reactive groups per tRNAPhe molecule. Under irradiation the derivatives modify exclusively the beta-subunit of the phenylalanyl-tRNA synthetase.
通过用4-(N-2-氯乙基-N-甲氨基)苄胺对苯丙氨酸转运核糖核酸（大肠杆菌）进行烷基化，随后用1,4-二硝基-5-氟苯叠氮化物处理，合成了苯丙氨酸转运核糖核酸的光反应性衍生物。当修饰程度低于每个苯丙氨酸转运核糖核酸分子3个反应基团时，这些衍生物在与核糖体和苯丙氨酰转运核糖核酸合成酶结合方面具有活性。在辐照下，这些衍生物专门修饰苯丙氨酰转运核糖核酸合成酶的β亚基。
