Torbay N, Bracco E F, Geliebter A, Stewart I M, Hashim S A
Am J Physiol. 1985 Jan;248(1 Pt 2):R120-4. doi: 10.1152/ajpregu.1985.248.1.R120.
The effect of experimentally induced hyperinsulinemia on body composition was studied in rats with food intakes precisely controlled by intragastric feeding and physical activity manipulated by sedation with chlordiazepoxide (CDP). Male Sprague-Dawley rats (n = 38) were fitted surgically with gastrostomy tubes. After 8 days the animals were divided into four groups. Group 1 received daily injections of protamine-zinc insulin; group 2 received daily injections of saline; group 3 received the same insulin doses as group 1 plus daily administration of CDP mixed with the diet; group 4 received daily injections of saline plus CDP in the diet. All groups were tubefed identical amounts of semiliquid diet via gastrostomy. Physical activity was measured by electronic monitor. After 4 wk the rats were killed. The insulin-treated groups (1 and 3) had significantly larger fat depots and larger mean fat cell size than the noninsulin-treated groups (2 and 4). This increase in fat occurred concurrently with a decrease in carcass protein and water. Physical activity, as measured, was unaltered by insulin but was significantly reduced by CDP. Treatment with CDP only increased the dorsal fat depot and liver weight but had no significant effect on total dissected fat depots and had a reductive effect on carcass protein. In conclusion insulin treatment enhanced the efficiency of conversion of energy intake into fat energy stores.
通过胃内喂养精确控制食物摄入量，并使用氯氮卓（CDP）镇静来控制身体活动，研究实验性诱导的高胰岛素血症对大鼠身体组成的影响。雄性Sprague-Dawley大鼠（n = 38）通过手术安装胃造口管。8天后，将动物分为四组。第1组每天注射精蛋白锌胰岛素；第2组每天注射生理盐水；第3组接受与第1组相同剂量的胰岛素，外加每天将CDP与饮食混合给药；第4组每天注射生理盐水并在饮食中添加CDP。所有组均通过胃造口管喂食等量的半流质饮食。通过电子监测仪测量身体活动。4周后处死大鼠。胰岛素治疗组（1和3）比未接受胰岛素治疗的组（2和4）有明显更大的脂肪储备和更大的平均脂肪细胞大小。脂肪的增加与胴体蛋白质和水分的减少同时发生。所测量的身体活动不受胰岛素影响，但CDP使其显著降低。仅用CDP治疗会增加背部脂肪储备和肝脏重量，但对总解剖脂肪储备没有显著影响，并且对胴体蛋白质有减少作用。总之，胰岛素治疗提高了能量摄入转化为脂肪能量储存的效率。
