Hainan Key Laboratory of Tropical Microbe Resources, Institute of Tropical Bioscience and Biotechnology, Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Hainan Institute for Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China.
J Agric Food Chem. 2024 Jun 12;72(23):13164-13174. doi: 10.1021/acs.jafc.4c02805. Epub 2024 May 31.
Obtaining a microorganism strain with a broad-spectrum resistance property and highly efficient antifungal activity is important to the biocontrol strategy. Herein, a marine sp. HNBCa1 demonstrated a broad-spectrum resistance to 17 tested crop pathogenic fungi and exhibited a high biocontrol efficiency against mango anthracnose and banana fusarium wilt. To uncover the critical bioactive secondary metabolites basis, genome assembly and annotation, metabolomic analysis, and a semipreparative HPLC-based activity-guide method were employed. Finally, geldanamycin and ectoine involved in codifferential secondary metabolites were also found to be related to biosynthetic gene clusters in the genome of HNBCa1. Reblastatin and geldanamycin were uncovered in response to broad-spectrum resistance to the 17 crop pathogenic fungi. Our results suggested that reblastatin and geldanamycin were critical to maintaining the broad-spectrum resistance property and highly efficient antifungal activity of HNBCa1, which could be further developed as a biological control agent to control crop fungal diseases.
获得具有广谱抗性和高效抗真菌活性的微生物菌株对于生物防治策略非常重要。本文报道了一株海洋 sp. HNBCa1，该菌株对 17 种作物病原真菌表现出广谱抗性，并对芒果炭疽病和香蕉枯萎病具有较高的生防效率。为了揭示关键的生物活性次生代谢物基础，我们采用了基因组组装和注释、代谢组学分析以及基于半制备 HPLC 的活性导向方法。最后，发现geldanamycin 和 ectoine 参与了 codifferential 次生代谢物的合成，并且与 HNBCa1 基因组中的生物合成基因簇有关。在对 17 种作物病原真菌的广谱抗性中发现了 reblastatin 和 geldanamycin。我们的结果表明，reblastatin 和 geldanamycin 对 HNBCa1 保持广谱抗性和高效抗真菌活性至关重要，可进一步开发为防治作物真菌病害的生物防治剂。
