Green T J, Gadsden G, Seed T, Jacobs R, Morhardt M, Brackett R
Am J Trop Med Hyg. 1985 Jan;34(1):24-30. doi: 10.4269/ajtmh.1985.34.24.
The FCR-3/FMG African strain of Plasmodium falciparum was cloned by the limiting dilution technique in in vitro culture to obtain parasite populations derived from a single cell. The basic in vitro culture technique used was that of Trager and Jensen, performed in microtiter culture plates. The cloning sequence was repeated serially three times, and three parasite clones with a higher than 99% probability of single cell derivation were isolated. Two of these clones were determined by electron microscopic examination to possess the K+ and one the K- trait. The clones were found to be equally sensitive to chloroquine in vitro but varied in their in vivo pathogenicity for Aotus, the K- clone being non-pathogenic.
采用有限稀释技术在体外培养中对恶性疟原虫FCR-3/FMG非洲株进行克隆，以获得源自单个细胞的寄生虫群体。所使用的基本体外培养技术是Trager和Jensen的方法，在微量培养板中进行。克隆过程连续重复三次，分离出三个单细胞衍生概率高于99%的寄生虫克隆。通过电子显微镜检查确定其中两个克隆具有K+特性，一个具有K-特性。发现这些克隆在体外对氯喹同样敏感，但对绢毛猴的体内致病性有所不同，K-克隆无致病性。
