Chernausek S D, Chatelain P G, Svoboda M E, Underwood L E, Van Wyk J J
Biochem Biophys Res Commun. 1985 Jan 16;126(1):282-8. doi: 10.1016/0006-291x(85)90603-5.
Somatomedin-C/insulin-like growth factor I was purified from human plasma using a monoclonal antibody affinity column. Combining immunoaffinity chromatography with standard protein purification methods resulted in an overall recovery of 18%. The 35 micrograms of somatomedin-C/insulin-like growth factor I purified from 500 ml of plasma appeared as a single band when analyzed by polyacrylamide gel electrophoresis and could be used in radioimmunoassay and receptor binding studies.
