Póvoa G, Wennberg G, Hall K
Biochem Biophys Res Commun. 1986 Apr 14;136(1):253-9. doi: 10.1016/0006-291x(86)90902-2.
A simplified procedure has been developed for the isolation of insulin-like growth factor I from human plasma by use of affinity chromatography with the somatomedin binding protein. After acidification of human plasma and separation of insulin-like growth factor I and endogenous binding protein by cation exchange chromatography on SP-Sephadex the material was passed through a column packed with pure human amniotic fluid binding protein covalently coupled to Sepharose. The bound insulin-like growth factors I and II were eluted by 1M acetic acid and separated on a Mono S cation exchange column by use of a salt gradient. The 30 micrograms insulin-like growth factor I and 18 micrograms insulin-like growth factor II recovered from 1 liter plasma gave an overall recovery of 30% for insulin-like growth factor I but only 2.5% for insulin-like growth factor II.
已开发出一种简化程序，通过使用与生长调节素结合蛋白的亲和色谱法从人血浆中分离胰岛素样生长因子I。将人血浆酸化后，通过在SP-葡聚糖凝胶上进行阳离子交换色谱法分离胰岛素样生长因子I和内源性结合蛋白，然后将该物质通过填充有与琼脂糖共价偶联的纯人羊水结合蛋白的柱子。结合的胰岛素样生长因子I和II用1M乙酸洗脱，并通过使用盐梯度在Mono S阳离子交换柱上进行分离。从1升血浆中回收的30微克胰岛素样生长因子I和18微克胰岛素样生长因子II，胰岛素样生长因子I的总回收率为30%，而胰岛素样生长因子II仅为2.5%。
