Houdijk W P, Sixma J J
Blood. 1985 Mar;65(3):598-604.
The role of subendothelial fibronectin in platelet interaction with subendothelium was studied. Human umbilical artery subendothelium was exposed to flowing blood containing 111In-labeled platelets in an annular perfusion chamber. Platelet adhesion was determined from the 111In radioactivity on the vessel wall. When perfusions were performed for five minutes at a wall shear rate of 1,800 s-1, platelet adhesion was the same whether normal plasma or fibronectin-free plasma was used. Preincubation of subendothelium with rabbit anti-human fibronectin serum, however, resulted in a marked inhibition of platelet adhesion. Preincubation with normal rabbit serum had no effect. Platelet adhesion was also diminished when the vessel wall was preincubated with anti-fibronectin IgG fraction or F(ab')2 fragment. After the latter preincubations, frozen sections of 4 micron were incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG, F(ab')2 fragment specific. Fluorescence was seen throughout the subendothelium both before and after perfusion. No fluorescence was seen when subendothelium was preincubated with normal rabbit IgG or F(ab')2 or with anti-fibronectin IgG that had been absorbed with purified fibronectin. After absorption of anti-fibronectin IgG with purified fibronectin, the inhibiting effect on platelet adhesion was also no longer present. Preincubation of the vessel wall with anti-fibronectin IgG reduced platelet adhesion significantly at a wall shear rate of 800 s-1. This effect was even greater at 1,800 s-1. At low shear rate (400 s-1), there was no inhibition.
研究了内皮下层纤连蛋白在血小板与内皮下层相互作用中的作用。在环形灌注室中，将人脐动脉内皮下层暴露于含有111In标记血小板的流动血液中。根据血管壁上的111In放射性测定血小板黏附情况。当在壁剪切速率为1800 s-1的条件下进行5分钟灌注时，无论使用正常血浆还是无纤连蛋白血浆，血小板黏附情况相同。然而，用兔抗人纤连蛋白血清对内皮下层进行预孵育，会导致血小板黏附显著受到抑制。用正常兔血清预孵育则无影响。当血管壁用抗纤连蛋白IgG组分或F(ab')2片段进行预孵育时，血小板黏附也会减少。在进行后者的预孵育后，将4微米厚的冰冻切片与异硫氰酸荧光素偶联的山羊抗兔IgG、F(ab')2片段特异性抗体一起孵育。在灌注前后，在内皮下层均可见荧光。当内皮下层用正常兔IgG或F(ab')2或用已被纯化纤连蛋白吸收的抗纤连蛋白IgG进行预孵育时，未见荧光。在用纯化纤连蛋白吸收抗纤连蛋白IgG后，对血小板黏附的抑制作用也不再存在。在壁剪切速率为800 s-1时，用抗纤连蛋白IgG对血管壁进行预孵育可显著降低血小板黏附。在1800 s-1时，这种作用甚至更大。在低剪切速率（400 s-1）下，没有抑制作用。
