Raska I, Kostrouch Z, Habrová V, Nedvídek J, Zatsepina O V, Neuzil A, Trebichavský I, Koukal M, Smetana K
Cesk Patol. 1985 Feb;21(1):28-37.
Colloid gold stabilized by macromolecules with various binding characteristics proved to be usable in transmission electron microscopy: Nucleic acids localization was studied in tissue cultures after embedding by techniques autoimmune serum-protein A--gold and RNase--gold. Technique with serum autoantibodies against double-stranded DNA resulted in marking condensed chromatin. Further experiments with ultrastructural localization of other autoimmune components seemed to be perspective and of diagnostic importance. Technique with RNase--gold complex marked especially ribosomes, nucleolus and interchromatin nuclear spaces. Herpes virus, rotavirus and enterovirus were identified in negative contrast by the technique antivirus serum--protein A--gold. A higher warrant of evidence achieved by the method may be used in virologic diagnosis. A small amount of tubulin was found in isolated permeabilized nuclei of Xenopus laevis by a direct immunocytochemical method with complex monoclonal IgG against tubulin-gold. Binding of complex of triiodothyronine-bovine serum albumin--gold on the cytoplasmic membrane of LEP cells in a short term tissue culture showed a possibility of tracing non-peptidic hormones binding on specific receptors.
事实证明，由具有各种结合特性的大分子稳定的胶体金可用于透射电子显微镜检查：在用自身免疫血清 - 蛋白A - 金和核糖核酸酶 - 金技术包埋后，研究了组织培养物中的核酸定位。针对双链DNA的自身抗体技术导致浓缩染色质被标记。其他自身免疫成分超微结构定位的进一步实验似乎具有前景且具有诊断重要性。核糖核酸酶 - 金复合物技术特别标记了核糖体、核仁和染色质间核空间。通过抗病毒血清 - 蛋白A - 金技术在负反差中鉴定出疱疹病毒、轮状病毒和肠道病毒。该方法获得的更高证据保证可用于病毒学诊断。通过针对微管蛋白 - 金的复合单克隆IgG的直接免疫细胞化学方法，在非洲爪蟾分离的通透核中发现了少量微管蛋白。在短期组织培养中，三碘甲状腺原氨酸 - 牛血清白蛋白 - 金复合物与LEP细胞的细胞质膜结合，显示出追踪非肽类激素与特异性受体结合的可能性。
