Donnenberg A D, Cameron J
Exp Hematol. 1985 Jan;13(1):29-35.
Time-lapse cinemicroscopy was used to observe the development of lymphocyte colonies in a phytohemagglutinin-dependent one-step, two-layer agar culture system. More than 1000 h of culture time were recorded in a total of 14 independent experiments. Blast formation and organization of lymphocytes into highly motile pairs and clusters occurred early in culture (0-3 days). An increase in thymidine uptake also preceded the first detectable proliferation by 24 h. Mature lymphocyte colonies were found to be dynamic entities characterized by the continuous influx and egress of highly motile cells. Cell clusters and entire colonies were observed to locomote and on several occasions fuse to form larger structures. Macrophagelike cells located centrally within colonies appeared to play a major role in such behavior. Taken together these results conclusively demonstrate that, in the present system, lymphocyte colonies are the product of a complex pattern of cell interaction, proliferation, and cell motility and, as such, are polyclonal in origin.
采用延时电影显微镜术观察淋巴细胞集落在植物血凝素依赖的一步两层琼脂培养系统中的发育情况。在总共14个独立实验中记录了超过1000小时的培养时间。培养早期（0 - 3天）就出现了母细胞形成以及淋巴细胞组织成高度运动的双细胞对和细胞簇。胸苷摄取增加也比首次可检测到的增殖提前24小时。发现成熟淋巴细胞集落是动态实体，其特征是高运动性细胞持续流入和流出。观察到细胞簇和整个集落会移动，并且有几次融合形成更大的结构。位于集落中心的巨噬样细胞似乎在这种行为中起主要作用。综合这些结果确凿地表明，在本系统中，淋巴细胞集落是细胞相互作用、增殖和细胞运动复杂模式的产物，因此起源是多克隆的。
