College of Food Science and Engineering, Ocean University of China, Sansha Road 1299, Qingdao, 266404, PR China.
Department of Nutrition and Food Hygiene, School of Public Health, Qingdao University, Ning Xia Road 308, Qingdao, 266071, PR China.
Food Chem. 2024 Oct 15;455:139882. doi: 10.1016/j.foodchem.2024.139882. Epub 2024 May 29.
A common epitope (AGSFDHKKFFKACGLSGKST) of parvalbumin from 16 fish species was excavated using bioinformatics tools combined with the characterization of fish parvalbumin binding profile of anti-single epitope antibody in this study. A competitive enzyme-linked immunosorbent assay (ELISA) based on the common epitope was established with a limit of detection of 10.15 ng/mL and a limit of quantification of 49.29 ng/mL. The developed ELISA exhibited a narrow range (71% to 107%) of related cross-reactivity of 15 fish parvalbumin. Besides, the recovery, the coefficient of variations for the intra-assay and the inter-assay were 84.3% to 108.2%, 7.4% to 13.9% and 8.5% to 15.6%. Our findings provide a novel idea for the development of a broad detection method for fish allergens and a practical tool for the detection of parvalbumin of economic fish species in food samples.
利用生物信息学工具，结合本研究中抗单表位抗体对鱼类 parvalbumin 结合谱的特征，挖掘了来自 16 种鱼类的 parvalbumin 的一个共同表位（AGSFDHKKFFKACGLSGKST）。建立了基于共同表位的竞争酶联免疫吸附测定（ELISA），检测限为 10.15ng/mL，定量限为 49.29ng/mL。开发的 ELISA 对 15 种鱼类 parvalbumin 的相关交叉反应具有较窄的范围（71%至 107%）。此外，回收率、内和间分析的变异系数分别为 84.3%至 108.2%、7.4%至 13.9%和 8.5%至 15.6%。我们的研究结果为开发鱼类过敏原的广谱检测方法提供了新的思路，为食品样品中经济鱼类 parvalbumin 的检测提供了实用工具。
