Centre for Quantum Physics, Key Laboratory of Advanced Optoelectronic Quantum Architecture and Measurement (MOE), School of Physics, Beijing Institute of Technology, Beijing 100081, China; International Center for Quantum Materials, Beijing Institute of Technology, Zhuhai, 519000, China; Beijing Key Lab of Nanophotonics and Ultrafine Optoelectronic Systems, School of Physics, Beijing Institute of Technology, Beijing 100081, China.
Key Laboratory of Molecular Medicine and Biological Diagnosis and Treatment (Ministry of Industry and Information Technology), School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Bioelectrochemistry. 2024 Oct;159:108748. doi: 10.1016/j.bioelechem.2024.108748. Epub 2024 May 26.
In this study, we have designed an electrochemical biosensor based on topological material BiSe for the sensitive detection of SARS-CoV-2 in the COVID-19 pandemic. Flake-shaped BiSe was obtained directly from high-quality single crystals using mechanical exfoliation, and the single-stranded DNA was immobilized onto it. Under optimal conditions, the peak current of the differential pulse voltammetry method exhibited a linear relationship with the logarithm of the concentration of target-complementary-stranded DNA, ranging from 1.0 × 10 to 1.0 × 10 M, with a detection limit of 3.46 × 10 M. The topological material BiSe, with Dirac surface states, enhanced the signal-to-interference plus noise ratio of the electrochemical measurements, thereby improving the sensitivity of the sensor. Furthermore, the electrochemical sensor demonstrated excellent specificity in recognizing RNA. It can detect complementary RNA by amplifying and transcribing the initial DNA template, with an initial DNA template concentration ranging from 1.0 × 10 to 1.0 × 10 M. Furthermore, the sensor also effectively distinguished negative and positive results by detecting splitting-synthetic SARS-CoV-2 pseudovirus with a concentration of 1 copy/μL input. Our work underscores the immense potential of the electrochemical sensing platform based on the topological material BiSe in the detection of pathogens during the rapid spread of acute infectious diseases.
在这项研究中，我们设计了一种基于拓扑材料 BiSe 的电化学生物传感器，用于在 COVID-19 大流行期间对 SARS-CoV-2 进行敏感检测。采用机械剥离法直接从高质量单晶中获得片状 BiSe，并将单链 DNA 固定在其上。在最佳条件下，差分脉冲伏安法的峰值电流与目标互补链 DNA 浓度的对数呈线性关系，范围从 1.0×10 到 1.0×10 M，检测限为 3.46×10 M。拓扑材料 BiSe 具有狄拉克表面态，增强了电化学测量的信噪比，从而提高了传感器的灵敏度。此外，电化学传感器在识别 RNA 方面表现出优异的特异性。它可以通过放大和转录初始 DNA 模板来检测互补 RNA，初始 DNA 模板浓度范围为 1.0×10 到 1.0×10 M。此外，该传感器还通过检测输入浓度为 1 拷贝/μL 的分裂合成 SARS-CoV-2 假病毒，有效地对阴性和阳性结果进行区分。我们的工作强调了基于拓扑材料 BiSe 的电化学传感平台在急性传染病迅速传播期间检测病原体方面的巨大潜力。
