Laboratory of Biotechnology and Physiology of Reproduction (LABIREP), Federal University of Ceará, Sobral, CE, Brazil.
Nucleus of Studies in Bioactive Phytochemicals (NEFB), Vale do Acaraú State University, Sobral, Ceará, Brazil.
Anim Reprod Sci. 2024 Jul;266:107514. doi: 10.1016/j.anireprosci.2024.107514. Epub 2024 May 29.
This study aims to investigate the influence of thymol on primordial follicle growth and survival, as well as on collagen fibers and stromal cells density in bovine ovarian tissues cultured in vitro. The activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), the thiol levels and the expression of mRNAs for SOD1, CAT, periredoxin 6 (PRDX6) and GPX1 were also investigated. Ovarian cortical tissues were cultured in α-MEM alone or with thymol (400, 800, 1600 or 3200 μg/mL) for six days. Before and after culture, the tissues were processed for histological analysis to evaluate follicular activation, growth, morphology, ovarian stromal cell density and collagen fibers. The levels of mRNA for SOD1, CAT, GPX1 and PRDX6 were evaluated by real-time PCR. The results show that tissues cultured with thymol (400 and 800 µg/mL) had increased percentages of normal follicles, when compared to tissues cultured in other treatments. At concentrations of 400 and 800 µg/mL, thymol maintained the rate of normal follicles similar to the uncultured control. In addition, 400 µg/mL thymol increased follicle activation, collagen fibers and stromal cell density of when compared to tissues cultured in control medium. The presence of 800 µg/mL thymol in culture medium increased CAT activity, while 400 or 800 µg/mL thymol reduced mRNA levels for SOD1, CAT and PRDX6, but did not alter GPX1 expression. In conclusion, 400 µg/mL thymol increases primordial follicle activation, preserves stromal cells, collagen fibers, and down-regulates expression of mRNA for SOD1, CAT and PRDX6 in cultured bovine ovarian tissues.
本研究旨在探讨百里香酚对体外培养牛卵巢组织中原始卵泡生长和存活以及胶原纤维和基质细胞密度的影响。还研究了超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性、巯基水平以及 SOD1、CAT、periredoxin 6（PRDX6）和 GPX1 的 mRNA 表达。将卵巢皮质组织在 α-MEM 中单独培养或与百里香酚（400、800、1600 或 3200μg/mL）一起培养 6 天。在培养前后，对组织进行组织学分析以评估卵泡激活、生长、形态、卵巢基质细胞密度和胶原纤维。通过实时 PCR 评估 SOD1、CAT、GPX1 和 PRDX6 的 mRNA 水平。结果表明，与在其他处理中培养的组织相比，用百里香酚（400 和 800μg/mL）培养的组织中正常卵泡的百分比增加。在 400 和 800μg/mL 浓度下，百里香酚维持正常卵泡的比率与未培养对照相似。此外，与在对照培养基中培养的组织相比，400μg/mL 百里香酚增加了卵泡激活、胶原纤维和基质细胞密度。培养基中存在 800μg/mL 百里香酚增加了 CAT 活性，而 400 或 800μg/mL 百里香酚降低了 SOD1、CAT 和 PRDX6 的 mRNA 水平，但不改变 GPX1 的表达。总之，400μg/mL 百里香酚增加了原始卵泡的激活，保留了基质细胞、胶原纤维，并下调了培养牛卵巢组织中 SOD1、CAT 和 PRDX6 的 mRNA 表达。
