da Silva Felipe F, das Chagas Costa Francisco, Azevedo Venância A N, de Assis Ernando I T, Gomes Geovany A, Araújo Valdevane R, de Morais Selene M, Rodrigues Tigressa H S, Silva José R V
Laboratory of Biotechnology and Physiology of Reproduction, Federal University of Ceara, Sobral, State of Ceará, 62042-280Brazil.
Laboratory of Phytochemical and Bioactive Products, State University Vale do Acarau, Sobral, Ceara, 62010-295Brazil.
J Pharm Pharmacol. 2024 Dec 2;76(12):1609-1619. doi: 10.1093/jpp/rgae093.
This study aims to evaluate the effects of Croton grewioides essential oil (CGEO) and anethole on follicle survival, growth, and oxidative stress in cultured bovine ovarian tissues.
Ovarian tissues were cultured for 6 days in a medium supplemented with different concentrations (1, 10, 100, or 1000 µg mL-1) of CGEO or anethole and then, follicular survival and growth, collagen content, and stromal cell density in ovarian tissues cultured in vitro were evaluated by histology. The mRNA levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase 1 (GPX1), peroxirredoxin 6 (PRDX6), and nuclear factor erythroid 2-related factor 2 (NRF2) were evaluated by real-time PCR. The activity of SOD, CAT, glutathione peroxidase (GPx), and thiol concentrations were investigated.
Ovarian tissues cultured with 1 µg mL-1 CGEO or anethole had a higher percentage of healthy follicles than those cultured in a control medium (P < .05). The 1 µg mL-1 CGEO also increased the number of stromal cells, collagen fibers, and thiol levels. Anethole (1 µg mL-1) increased CAT activity and reduced that of GPx. The activity of SOD was reduced by CGEO. In contrast, 1 µg mL-1 anethole reduced mRNA for CAT, PRDX1, and NRF2 (P < .05). In addition, 1 µg mL-1 CGEO reduced mRNA for CAT, PRDX6, and GPx1 (P < .05).
The presence of 1 µg mL-1 anethole or CGEO in a culture medium promotes follicle survival and regulates oxidative stress and the expression of mRNA and activity of antioxidant enzymes in cultured bovine ovarian tissues.
本研究旨在评估巴豆（Croton grewioides）精油（CGEO）和茴香脑对培养的牛卵巢组织中卵泡存活、生长及氧化应激的影响。
将卵巢组织在添加不同浓度（1、10、100或1000 µg mL-1）CGEO或茴香脑的培养基中培养6天，然后通过组织学评估体外培养的卵巢组织中的卵泡存活与生长、胶原蛋白含量及基质细胞密度。通过实时PCR评估超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶1（GPX1）、过氧化物还原酶6（PRDX6）和核因子红细胞2相关因子2（NRF2）的mRNA水平。研究SOD、CAT、谷胱甘肽过氧化物酶（GPx）的活性及硫醇浓度。
用1 µg mL-1 CGEO或茴香脑培养的卵巢组织中，健康卵泡的百分比高于在对照培养基中培养的组织（P <.05）。1 µg mL-1 CGEO还增加了基质细胞数量、胶原纤维和硫醇水平。茴香脑（1 µg mL-1）增加了CAT活性并降低了GPx活性。CGEO降低了SOD活性。相比之下，1 µg mL-1茴香脑降低了CAT、PRDX1和NRF2的mRNA水平（P <.05）。此外，1 µg mL-1 CGEO降低了CAT、PRDX6和GPX1的mRNA水平（P <.05）。
培养基中存在1 µg mL-1茴香脑或CGEO可促进卵泡存活，并调节培养的牛卵巢组织中的氧化应激以及抗氧化酶的mRNA表达和活性。
