Department of Pharmacy, College of Medicine and Health Sciences, Debre Markos University, Northwest, Ethiopia.
Department of Pharmacy, College of Medicine and Health Sciences, Debre Markos University, Northwest, Ethiopia.
J Ethnopharmacol. 2024 Oct 28;333:118413. doi: 10.1016/j.jep.2024.118413. Epub 2024 May 31.
Novel drugs are needed to address the issue of malarial infection resistance; natural items can be a different source of these medications. Albizia malacophylla (A. Rich.) Walp. (Leguminosae) is listed as one of the antimalarial medicinal plants in Ethiopian folk medicine. However, there are no reports regarding the biological activity or phytochemistry of the plant.
Thus, this study aimed to evaluate the A. malacophylla crude extract and solvent fractions' in vivo antimalarial activity utilizing 4-day suppressive, preventative, and curative tests in mice infected with P. berghei.
The parasite Plasmodium berghei, which causes rodent malaria, was used to infect healthy male Swiss Albino mice, weighing 23-28 g and aged 6-8 weeks. Solvent fractions such as methanol, water, and chloroform were given in addition to an 80% methanolic extract at 100, 200, and 400 mg/kg doses. A Conventional test such as parasitemia, survival time, body weight, temperature, and packed cell capacity were employed to ascertain factors such as the suppressive, curative, and preventive tests.
Every test substance dramatically reduced the number of parasites in every experiment. Crude extract (with the highest percentage suppression of 67.78%) performs better antimalarial effect than the methanol fraction, which is the most efficient solvent fraction with a percentage suppression of 55.74%. With a suppression value of 64.83% parasitemia level, the therapeutic effects of 80% methanolic crude extract were greater than its curative and preventative effects in a four-day suppressive test. The survival period (17 days) was longer with the hydroalcoholic crude extract dose of 400 mg/kg than with other doses of the materials under investigation.
The results of this investigation validate the antimalarial characteristics of A. malacophylla leaf extract. The crude extract prevented weight loss, a decline in temperature, and a reduction in PCV. The results demonstrate that the plant has a promising antimalarial effect against P. berghei, hence supporting the traditional use of the plant. Therefore, it could serve as a foundation for the development of new antimalarial drugs.
需要新型药物来解决疟疾感染耐药性问题；天然物质可能是这些药物的另一个来源。合欢（A. Rich.）Walp.（豆科）被列为埃塞俄比亚民间医学中的抗疟药用植物之一。然而，关于该植物的生物活性或植物化学尚无报道。
因此，本研究旨在利用 4 天抑制、预防和治疗试验，评估合欢粗提取物和溶剂级分在感染伯氏疟原虫的小鼠体内的抗疟活性。
使用导致啮齿动物疟疾的寄生虫伯氏疟原虫感染健康雄性瑞士白化病小鼠，体重 23-28g，年龄 6-8 周。除了给予 80%甲醇提取物 100、200 和 400mg/kg 剂量外，还给予甲醇、水和氯仿等溶剂级分。采用常规试验如寄生虫血症、生存时间、体重、体温和红细胞压积，以确定抑制、治疗和预防试验等因素。
每种试验物质都显著降低了每个实验中的寄生虫数量。粗提取物（抑制率最高达 67.78%）比甲醇级分表现出更好的抗疟效果，甲醇级分是最有效的溶剂级分，抑制率为 55.74%。在 4 天抑制试验中，80%甲醇粗提取物的治疗效果抑制率为 64.83%，高于其预防和治疗效果。与研究材料的其他剂量相比，400mg/kg 剂量的水醇粗提取物的生存时间（17 天）更长。
本研究结果验证了合欢叶提取物的抗疟特性。粗提取物预防了体重减轻、体温下降和 PCV 下降。结果表明，该植物对伯氏疟原虫具有有前途的抗疟作用，因此支持该植物的传统用途。因此，它可以作为开发新的抗疟药物的基础。
