感染疟原虫的小鼠中叶片的氢甲醇提取物及其溶剂提取物的抗疟活性。 不过你提供的原文中“infected with.”后面缺少具体感染的对象，翻译可能会稍显不完整。
Antimalarial activity of hydromethanolic extract and its solvent fractions of leaves in mice infected with .
作者信息
Bihonegn Temesgen, Giday Mirutse, Yimer Getnet, Animut Abebe, Sisay Mekonnen
机构信息
Department of Animal Health, Kombolcha Agricultural College, Kombolcha, Ethiopia.
Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.
出版信息
SAGE Open Med. 2019 May 13;7:2050312119849766. doi: 10.1177/2050312119849766. eCollection 2019.
BACKGROUND
Del. (Asteraceae) is reported to be traditionally used for the treatment of malaria. Based on folkloric repute of this plant in Ethiopian traditional medicine and crude extract-based ethnopharmacological studies conducted in few countries, this study was undertaken to evaluate the in vivo antimalarial activity of 80% methanol extract and its solvent fractions of the leaves of in mice infected with .
METHODS
A 4-day suppressive test was conducted on mice infected with to find out antimalarial effect of chloroform, butanol and aqueous fractions obtained from the 80% methanol crude extract. In all the activity tests, mice were randomly assigned in five groups (three tests and two controls) of six animals in each and received respective treatments. Data were analyzed using one way analysis of variance followed by Tukey's post hoc test for multiple comparisons.
RESULTS
Acute oral toxicity test showed that all solvent fractions of the leaves of revealed neither mortality nor overt signs of toxicity up to 2000 mg/kg. This study indicated that the percentage parasitemia suppression of 80% methanol extract was 32.47% (±2.65), 35.40% (±3.14) and 37.67% (±2.50) at 200, 400 and 600 mg/kg, respectively. All doses of the 80% methanol extract of prolonged survival time and prevented weight loss and packed cell volume reduction in infected mice. All doses of chloroform and butanol fractions significantly suppressed parasitemia (p < 0.05), increased survival time (p < 0.05) compared to negative control and exhibited a significant reduction in rectal temperature (p < 0.05). All solvent fractions significantly prevented weight loss (p < 0.05) at all tested doses. The 80% methanol extract and chloroform and butanol fractions significantly (p < 0.05) prevented further reduction in rectal temperature of infected mice at all doses.
CONCLUSION
The results of this study indicated that 80% methanol extract and solvent fractions of the leaves of demonstrated promising antimalarial activity. The study corroborated the folklore use of this plant for the treatment of malaria in ethnomedicine in Ethiopia.
背景
据报道，紫菀属（菊科）传统上用于治疗疟疾。基于这种植物在埃塞俄比亚传统医学中的民间声誉以及在少数国家进行的基于粗提物的民族药理学研究，本研究旨在评估80%甲醇提取物及其叶的溶剂萃取物对感染伯氏疟原虫的小鼠的体内抗疟活性。
方法
对感染伯氏疟原虫的小鼠进行为期4天的抑制试验，以确定从80%甲醇粗提物中获得的氯仿、丁醇和水相萃取物的抗疟效果。在所有活性测试中，将小鼠随机分为五组（三项试验和两项对照），每组六只动物，并接受相应处理。数据采用单因素方差分析，随后进行Tukey事后检验进行多重比较。
结果
急性经口毒性试验表明，紫菀属叶的所有溶剂萃取物在高达2000mg/kg时均未显示死亡率或明显的毒性迹象。本研究表明，80%甲醇提取物在200、400和600mg/kg时对疟原虫血症的抑制率分别为32.47%（±2.6）、35.40%（±3.14）和37.67%（±2.50）。80%甲醇提取物的所有剂量均延长了感染小鼠的存活时间，防止了体重减轻和红细胞压积降低。与阴性对照相比，氯仿和丁醇萃取物的所有剂量均显著抑制疟原虫血症（p<0.05），延长存活时间（p<0.05），并使直肠温度显著降低（p<0.05）。所有溶剂萃取物在所有测试剂量下均显著防止体重减轻（p<0.05）。80%甲醇提取物以及氯仿和丁醇萃取物在所有剂量下均显著（p<0.05）防止感染小鼠的直肠温度进一步降低。
结论
本研究结果表明，紫菀属叶的80%甲醇提取物及其溶剂萃取物具有良好的抗疟活性。该研究证实了这种植物在埃塞俄比亚民族医学中用于治疗疟疾的民间用途。
Zotero 插件