Hamelin C
Int J Radiat Oncol Biol Phys. 1985 Feb;11(2):253-7. doi: 10.1016/0360-3016(85)90146-4.
Agarose gel electrophoresis and electron microscopy were used to determine the type of lesions produced in DNA by ozone. This strong oxidizing agent was found to relax, linearize, then degrade native plasmid (pAT153) DNA molecules in solution. Ozone, like ionizing radiation, thus produced DNA breakage. To ascertain this point, wild-type and radiosensitive strains of Escherichia coli were transfected with control or ozonated plasmid DNA, and the host cells were selected for antibiotic resistance. A significant reduction in the transforming ability of pAT153 was observed following ozonation. Mutants deficient in the repair of DNA single-strand breaks yielded less ampicillin- or tetracycline-resistant clones than repair-proficient strains. In E. coli, the same gene products are probably involved in the repair of both radiation- and ozone-induced DNA breaks.
使用琼脂糖凝胶电泳和电子显微镜来确定臭氧对DNA造成的损伤类型。发现这种强氧化剂会使溶液中的天然质粒（pAT153）DNA分子先松弛、线性化，然后降解。因此，臭氧与电离辐射一样，会导致DNA断裂。为了确定这一点，用对照质粒DNA或经臭氧处理的质粒DNA转染大肠杆菌的野生型和辐射敏感菌株，并选择宿主细胞以获得抗生素抗性。臭氧处理后，观察到pAT153的转化能力显著降低。与修复能力正常的菌株相比，缺乏DNA单链断裂修复能力的突变体产生的氨苄青霉素或四环素抗性克隆较少。在大肠杆菌中，可能相同的基因产物参与修复辐射和臭氧诱导的DNA断裂。
