鼻息肉型慢性鼻-鼻窦炎中组蛋白表达降低。
Decreased histone expression in chronic rhinosinusitis with nasal polyps.
作者信息
Zhao Yanming, Zhang Nan, Perez Novo Claudina, Wang Yang, Zhang Luo
机构信息
Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, Beijing, China.
Upper Airways Research Laboratory (URL), Department of Oto-Rhino-Laryngology, Ghent University Hospital, Ghent, Belgium.
出版信息
Asia Pac Allergy. 2024 Jun;14(2):70-76. doi: 10.5415/apallergy.0000000000000140. Epub 2024 Apr 4.
BACKGROUND
Histones have been associated with human diseases. However, the implication of extranuclear histone proteins and their potential mechanism in the pathophysiology of chronic rhinosinusitis (CRS) have not been thoroughly investigated. This study was designed to evaluate the role of histones in patients with CRS by comparing histone expression between patients and controls.
METHODS
Nasal polyp (NP) tissues were obtained, and their comprehensive gene expression profiles were investigated by microarray analysis. Differences in expression were verified by reverse transcriptase polymerase chain reaction and immunohistochemical staining. Cell culture and flow cytometry were used to evaluate the role of histones in the pathogenesis of polyps.
RESULTS
Significant differences in the microarray analysis were observed between the patient and control groups ( < 0.01). It was found by flow cytometry that the histone (H2BK) can promote cell apoptosis in NPs.
CONCLUSION
Our results indicate that reduced expression of may contribute to the imbalance process of cell proliferation and apoptosis in CRS with NP.
背景
组蛋白与人类疾病有关。然而，核外组蛋白及其在慢性鼻-鼻窦炎（CRS）病理生理学中的潜在机制尚未得到充分研究。本研究旨在通过比较患者与对照组之间的组蛋白表达，评估组蛋白在CRS患者中的作用。
方法
获取鼻息肉（NP）组织，并通过微阵列分析研究其综合基因表达谱。通过逆转录聚合酶链反应和免疫组织化学染色验证表达差异。使用细胞培养和流式细胞术评估组蛋白在息肉发病机制中的作用。
结果
患者组与对照组之间在微阵列分析中观察到显著差异（<0.01）。通过流式细胞术发现组蛋白（H2BK）可促进NP中的细胞凋亡。
结论
我们的结果表明，[此处原文缺失具体组蛋白名称]表达降低可能导致伴NP的CRS中细胞增殖和凋亡的失衡过程。
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