Ali M, Nalebuff D J, Fayemi A O, Fadal R G, Connell J T
Otolaryngol Head Neck Surg (1979). 1979 Jan-Feb;87(1):47-51. doi: 10.1177/019459987908700113.
A solid-phase immunoenzymatic technique for the detection of allergen-specific IgE antibodies in serum is described. The binding of such antibodies to allergen insolubilized on cyanogen bromide-activated paper discs was detected by a subsequent two-step procedure involving the use of rabbit antihuman IgE and goat antirabbit IgG coupled with peroxidase. A solution of 3-3' diaminobenzidine and hydrogen peroxide, employed as the color indicator system, turned the discs dark brown in positive cases. Discs carrying 11 different inhalant allergens were tested with sera containing allergen-specific IgE antibodies. Agreement between the results of this technique and the RAST was seen in 85% of 310 tests performed. In the clinical practice of otorhinolaryngologic allergy, this test may prove to be an important laboratory adjunct ot clinical history and diagnostic skin test in the identification of the incriminated inhalant allergens.
本文描述了一种用于检测血清中变应原特异性IgE抗体的固相免疫酶技术。此类抗体与固定在溴化氰活化纸片上的变应原的结合，通过后续两步法检测，该方法使用与过氧化物酶偶联的兔抗人IgE和山羊抗兔IgG。用作颜色指示系统的3-3'二氨基联苯胺和过氧化氢溶液，在阳性病例中使纸片变为深棕色。用含有变应原特异性IgE抗体的血清检测携带11种不同吸入性变应原的纸片。在310次检测中，该技术与放射变应原吸附试验（RAST）结果的一致性为85%。在耳鼻喉科变应性疾病的临床实践中，该试验可能被证明是临床病史和诊断性皮肤试验的重要实验室辅助手段，有助于识别致病的吸入性变应原。
