Nakagawa T, Iwasaki E, Baba M, Matsushita T, Baba S, Ito K, Miyamoto T
Arerugi. 1989 Jun;38(6):478-85.
MAST, which stand for multiple antigen simultaneous test, uses enzyme-linked anti-human IgE and chemiluminogenic substate to determine IgE. This system is characterized by simultaneous analysis of multiple allergen items, up to 35, together with total IgE determination. We evaluated usefulness of this MAST system using 191 serum samples obtained from patients with bronchial asthma, allergic rhinitis and/or atopic dermatitis. It was found that there were statistically significant correlations between IgE antibody quantification by MAST and RAST in 24 out of 35 allergen items, with correlation coefficients more than r = 0.60. These included Dermatophagoides farinae and pteronyssinus, Japanese cedar pollen, orchard grass, Alternaria, Candida as inhalant allergens; egg white, milk, soybeans, wheat and rice as food allergens. It was also evaluated how many allergen-specific IgE antibodies could be detected in one serum sample. More than six allergen-specific IgE antibodies were simultaneously detected in 33% of 191 cases, indicating the importance of multiple-allergen analysis. These results indicate the clinical usefulness of the MAST allergy system in detecting IgE antibodies in allergic subjects.
MAST代表多重抗原同步检测，它利用酶联抗人IgE和化学发光底物来测定IgE。该系统的特点是能够同时分析多达35种的多种过敏原项目，并同时测定总IgE。我们使用从支气管哮喘、过敏性鼻炎和/或特应性皮炎患者中获取的191份血清样本，评估了该MAST系统的实用性。结果发现，在35种过敏原项目中的24种中，MAST测定的IgE抗体定量与RAST之间存在统计学上的显著相关性，相关系数r大于0.60。这些过敏原包括屋尘螨和粉尘螨、日本雪松花粉、果园草、链格孢属、念珠菌等吸入性过敏原；蛋清、牛奶、大豆、小麦和大米等食物过敏原。研究还评估了一份血清样本中能够检测到多少种过敏原特异性IgE抗体。在191例病例中的33%中同时检测到了六种以上的过敏原特异性IgE抗体，这表明多重过敏原分析的重要性。这些结果表明MAST过敏系统在检测过敏患者IgE抗体方面具有临床实用性。
