Al-Younis Zena Kadhim, Almajidi Yasir Qasim, Mansouri Sofiene, Ahmad Irfan, Turdialiyev Umid, O Alsaab Hashem, F Ramadan Montather, Joshi S K, Alawadi Ahmed Hussien, Alsaalamy Ali
Food Science Department, Agriculture College, Basrah University, Basrah, Iraq.
Department of Pharmacy (Pharmaceutics), Baghdad College of Medical Sciences, Baghdad, Iraq.
Crit Rev Anal Chem. 2024 Jun 3:1-22. doi: 10.1080/10408347.2024.2356842.
Field effect transistors (FETs)-based detection probes are powerful platforms for quantification in biological media due to their sensitivity, ease of miniaturization, and ability to function in biological media. Especially, FET-based platforms have been utilized as promising probes for label-free detections with the potential for use in real-time monitoring. The integration of new materials in the FET-based probe enhances the analytical performance of the developed probes by increasing the active surface area, rejecting interfering agents, and providing the possibility for surface modification. Furthermore, the use of new materials eliminates the need for traditional labeling techniques, providing rapid and cost-effective detection of biological analytes. This review discusses the application of materials in the development of FET-based label-free systems for point-of-care (POC) analysis of different biomedical analytes from 2018 to 2024. The mechanism of action of the reported probes is discussed, as well as their pros and cons were also investigated. Also, the possible challenges and potential for the fabrication of commercial devices or methods for use in clinics were discussed.
基于场效应晶体管（FET）的检测探针因其灵敏度高、易于小型化以及能够在生物介质中发挥作用，成为生物介质定量分析的强大平台。特别是，基于FET的平台已被用作有前景的无标记检测探针，具有用于实时监测的潜力。在基于FET的探针中集成新材料，通过增加活性表面积、排除干扰剂以及提供表面修饰的可能性，提高了所开发探针的分析性能。此外，新材料的使用消除了对传统标记技术的需求，能够快速且经济高效地检测生物分析物。本综述讨论了2018年至2024年材料在基于FET的无标记系统开发中的应用，该系统用于不同生物医学分析物的即时护理（POC）分析。文中讨论了所报道探针的作用机制，并对其优缺点进行了研究。此外，还讨论了用于临床的商业设备或方法制造可能面临的挑战和潜力。
