Sanz A, García-Barreno B, Nogal M L, Viñuela E, Enjuanes L
J Virol. 1985 Apr;54(1):199-206. doi: 10.1128/JVI.54.1.199-206.1985.
We have obtained 60 stable hybridomas which produced immunoglobulins that recognized 12 proteins from African swine fever virus particles and African swine fever virus-infected cells. Most of the monoclonal antibodies were specific for the three major structural proteins p150, p72, and p12. The specificity of some monoclonal antibodies for the structural proteins p150 and p37 and the nonstructural proteins p220 and p60 indicated that proteins p150 and p220 are antigenically related to proteins p37 and p60. The association of some viral antigens to specific subcellular components was determined by immunofluorescence and analysis of the binding of monoclonal antibodies to infected cells. A host protein (p24) seemed to be associated with the virus particles.
我们获得了60个稳定的杂交瘤，它们产生的免疫球蛋白能够识别非洲猪瘟病毒颗粒和感染非洲猪瘟病毒的细胞中的12种蛋白质。大多数单克隆抗体对三种主要结构蛋白p150、p72和p12具有特异性。一些单克隆抗体对结构蛋白p150和p37以及非结构蛋白p220和p60的特异性表明，蛋白质p150和p220在抗原性上与蛋白质p37和p60相关。通过免疫荧光和分析单克隆抗体与感染细胞的结合，确定了一些病毒抗原与特定亚细胞成分的关联。一种宿主蛋白（p24）似乎与病毒颗粒有关。
