Shikata H, Suzuki K, Henmi A, Ushiyama H, Uchida T, Utsumi N, Shikata T
Oral Surg Oral Med Oral Pathol. 1985 Jan;59(1):58-62. doi: 10.1016/0030-4220(85)90116-1.
Localization of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in the human parotid gland and liver was studied by an immunohistochemical method in four cases with seropositive HBsAg. Neither HBsAg nor HBcAg could be detected in the parenchymal cells of the parotid gland in any of the cases. However, one of four cases, which had the highest titer of serum HBsAg, showed HBsAg immunoreactivity in the vascular wall and luminal fluid of the parotid gland. In liver, HBsAg was detected in three and HBcAg in two of the four cases, respectively. HBsAg was localized in the cytoplasm of hepatocytes, while HBcAg was localized mainly in their nuclei. The detection of HBsAg in the parotid gland and liver was correlated with the serum titer of the antigen. The results indicate that the HB virus does not have an affinity for or a replicate in the parotid gland. Also, it is suggested that the HBsAg found in saliva is derived from HBsAg circulating through the oral mucosa by capillary leakage and not from secretion into the mouth by the salivary gland.
采用免疫组织化学方法对4例乙肝表面抗原（HBsAg）血清学阳性患者的腮腺和肝脏中HBsAg及乙肝核心抗原（HBcAg）进行定位研究。所有病例的腮腺实质细胞中均未检测到HBsAg和HBcAg。然而，在血清HBsAg滴度最高的4例病例中的1例，其腮腺血管壁和管腔内液显示出HBsAg免疫反应性。在肝脏中，4例病例中有3例检测到HBsAg，2例检测到HBcAg。HBsAg定位于肝细胞的细胞质中，而HBcAg主要定位于细胞核中。腮腺和肝脏中HBsAg的检测与抗原的血清滴度相关。结果表明，乙肝病毒对腮腺没有亲和力或不在腮腺中复制。此外，提示唾液中发现的HBsAg源自通过毛细血管渗漏经口腔黏膜循环的HBsAg，而非由唾液腺分泌至口腔。
