Shi Yuru, Zhang Xiaoqian, Pei Shengji, Wang Yuhua
Department of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences, Lanhei Road 132, Heilongtan, Kunming, 650201, Yunnan, China.
Nat Prod Bioprospect. 2024 Jun 4;14(1):36. doi: 10.1007/s13659-024-00458-8.
Adenosma buchneroides Bonati, also known as fleagrass, is an important medicinal plant used by the Akha (Hani) people of China for treating inflammation-related skin swelling, acne, and diarrhoea, among other conditions. In this study, we aimed to evaluate the anti-inflammatory activities and explore the molecular mechanisms of fleagrass on treating skin swelling and acne. The results demonstrated that fleagrass inhibited the enzymatic activities of 5-LOX and COX-2 in vitro, and decreased the release of NO, IL-6, TNF-α, and IL-10 in the LPS-induced RAW264.7 macrophages. The levels of proteins associated with the nuclear factor-kappa B (NF-κB) pathway were examined by western blotting and immunofluorescence, demonstrating that fleagrass downregulated the expression of TLR4, MyD88, NF-κB/p65, and iNOS and blocked the nuclear translocation of NF-κB/p65. Furthermore, fleagrass exhibited acute anti-inflammatory activity in paw oedema models. The results confirm that fleagrass exhibits remarkable anti-inflammatory activity and can be used in alleviating inflammation, suggesting that fleagrass has the potential to be a novel anti-inflammatory agent.
拟地皮消，又称跳蚤草，是中国阿卡（哈尼）族用于治疗炎症相关皮肤肿胀、痤疮和腹泻等多种病症的一种重要药用植物。在本研究中，我们旨在评估跳蚤草的抗炎活性，并探索其治疗皮肤肿胀和痤疮的分子机制。结果表明，跳蚤草在体外抑制了5-脂氧合酶（5-LOX）和环氧化酶-2（COX-2）的酶活性，并减少了脂多糖（LPS）诱导的RAW264.7巨噬细胞中一氧化氮（NO）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和白细胞介素-10（IL-10）的释放。通过蛋白质印迹法和免疫荧光法检测了与核因子-κB（NF-κB）信号通路相关的蛋白质水平，结果表明跳蚤草下调了Toll样受体4（TLR4）、髓样分化因子88（MyD88）、NF-κB/p65和诱导型一氧化氮合酶（iNOS）的表达，并阻断了NF-κB/p65的核转位。此外，跳蚤草在足爪肿胀模型中表现出急性抗炎活性。这些结果证实跳蚤草具有显著的抗炎活性，可用于减轻炎症，表明跳蚤草有潜力成为一种新型抗炎药物。
