基于聚合物/纸杂交微流控生物芯片的尿液多指标智能检测用于高尿酸血症监测。
Urine multi-index intelligent detection based on polymer/paper hybrid microfluidic biochip for hyperuricemia monitoring.
机构信息
Institute of Biomedical Precision Testing and Instrumentation, College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong, Shanxi, 030600, China.
Department of Endocrinology, The First Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030000, China; Academy of Medical Sciences, Shanxi Medical University, Taiyuan, Shanxi, 030000, China.
出版信息
Anal Chim Acta. 2024 Jul 11;1312:342742. doi: 10.1016/j.aca.2024.342742. Epub 2024 May 21.
Hyperuricemia (HUA) has gradually become a public health burden as an independent risk factor for a variety of chronic diseases. Herein, a user-friendly point-of-care (POC) detection system (namely "Smart-HUA-Monitor") based on smartphone-assisted paper-based microfluidic is proposed for colorimetric quantification of HUA urinary markers, including uric acid (UA), creatinine (CR) and pH. The detection limits of UA and CR were 0.0178 and 0.5983 mM, respectively, and the sensitivity of pH were 0.1. The method was successfully validated in artificial urine samples and 100 clinical samples. Bland-Altman plots showed a high consistency between μPAD and the testing instruments (HITACHI 7600 Automatic Analyzer, URIT-500B Urine Analyzer and AU5800B automatic biochemical analyzer) in hospital. Smart-HUA-Monitor provides an accurate quantitative, rapid, low-cost and reliable tool for the monitoring and early diagnosis of HUA urine indicators.
高尿酸血症（HUA）作为多种慢性病的独立危险因素，已逐渐成为公共卫生负担。在此，我们提出了一种基于智能手机辅助纸质微流控的简便型即时检测系统（即“Smart-HUA-Monitor”），用于对 HUA 尿标志物（包括尿酸（UA）、肌酐（CR）和 pH）进行比色定量检测。UA 和 CR 的检测限分别为 0.0178 和 0.5983 mM，pH 的灵敏度为 0.1。该方法已成功在人工尿液样本和 100 份临床样本中得到验证。 Bland-Altman 图显示 μPAD 与医院检测仪器（日立 7600 全自动分析仪、URIT-500B 尿液分析仪和 AU5800B 全自动生化分析仪）之间具有高度一致性。Smart-HUA-Monitor 为 HUA 尿液标志物的监测和早期诊断提供了一种准确、快速、低成本和可靠的定量工具。
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