Qiu Zijian, Pang Guanchao, Xu Xia, Lin Jun, Wang Pingli
Department of Radiation Oncology, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, Zhejiang, China.
Department of Respiratory and Critical Care Medicine, The Second Affiliated Hospital of Zhejiang University School of Medicine, No. 1511, Jianghong Road, Hangzhou, 310003, China.
Discov Oncol. 2024 Jun 4;15(1):208. doi: 10.1007/s12672-024-01062-5.
The role of mast cells in malignancies remains unclear, and there is no clear correlation between mast cells and tumor microvessels, tumor growth, or lung adenocarcinoma (LUAD) prognosis. This study aims to explore the association between mast cell density (MCD) and intratumoral microvessel density (MVD), clinicopathological parameters, and prognosis in LUAD, by evaluating mast cell infiltration characteristics and their prognostic significance.
This retrospective investigation involved 238 patients with LUAD undergoing complete resection. Tumor and normal lung tissue sections outside the tumor were immunohistochemically stained for MCD in the intratumoral and outside regions, respectively. CD34 polyclonal antibody was used to measure intratumoral MVD.
Intratumoral regions of LUAD had a higher MCD (P < 0.001) than normal lung tissue. In the intratumoral region, MCD and CD34-MVD were positively correlated (r = 0.411, P < 0.001). Intratumoral MCD correlated with sex, smoking history, tumor differentiation, pathological subtype, and tumor size. Female sex (P = 0.012), no smoking history (P = 0.002), acinar predominant type (P = 0.012), and tumor size ≤ 3 cm (P = 0.009) were associated with a higher MCD, whereas poorly differentiated (P = 0.039) and solid/micropapillary predominant types (P = 0.001) were associated with a lower MCD. Higher intratumoral MCD exhibited a marginally improved overall survival, and individuals with higher MCD infiltration ratios (intratumoral MCD/outside the MCD) had higher disease-free and overall survival rates (log-rank P < 0.001). A high MCD infiltration ratio was associated with decreased risk of tumor progression and death following complete resection.
The tumor microenvironment controls mast cell infiltration in LUAD, and patients with increased intratumoral mast cell infiltration have better prognosis.
肥大细胞在恶性肿瘤中的作用仍不清楚，且肥大细胞与肿瘤微血管、肿瘤生长或肺腺癌（LUAD）预后之间没有明确的相关性。本研究旨在通过评估肥大细胞浸润特征及其预后意义，探讨肥大细胞密度（MCD）与LUAD肿瘤内微血管密度（MVD）、临床病理参数及预后之间的关联。
本回顾性研究纳入了238例行根治性切除术的LUAD患者。分别对肿瘤组织及肿瘤外正常肺组织切片进行免疫组织化学染色，以检测肿瘤内及肿瘤外区域的MCD。采用CD34多克隆抗体检测肿瘤内MVD。
LUAD肿瘤内区域的MCD高于正常肺组织（P < 0.001）。在肿瘤内区域，MCD与CD34-MVD呈正相关（r = 0.411，P < 0.001）。肿瘤内MCD与性别、吸烟史、肿瘤分化程度、病理亚型及肿瘤大小相关。女性（P = 0.012）、无吸烟史（P = 0.002）、腺泡为主型（P = 0.012）及肿瘤大小≤3 cm（P = 0.009）与较高的MCD相关，而低分化（P = 0.039）及实体/微乳头为主型（P = 0.001）与较低的MCD相关。较高的肿瘤内MCD显示总生存期略有改善，且MCD浸润率较高（肿瘤内MCD/肿瘤外MCD）的个体无病生存期和总生存率较高（对数秩检验P < 0.00）。高MCD浸润率与根治性切除后肿瘤进展和死亡风险降低相关。
肿瘤微环境控制LUAD中肥大细胞的浸润，肿瘤内肥大细胞浸润增加的患者预后较好。
