Lee Seung Young, Lee Gileung, Han Jiheon, Ha Su-Kyung, Lee Chang-Min, Kang Kyeongmin, Jin Mina, Suh Jung-Pil, Jeung Ji-Ung, Mo Youngjun, Lee Hyun-Sook
Crop Breeding Division, National Institute of Crop Science, Rural Development Administration, Wanju, Republic of Korea.
Department of Crop Science and Biotechnology, Jeonbuk National University, Jeonju, Republic of Korea.
Front Plant Sci. 2024 May 21;15:1412614. doi: 10.3389/fpls.2024.1412614. eCollection 2024.
Rice blast is a destructive fungal disease affecting rice plants at various growth stages, significantly threatening global yield stability. Development of resistant rice cultivars stands as a practical means of disease control. Generally, association mapping with a diversity panel powerfully identifies new alleles controlling trait of interest. On the other hand, utilization of a breeding panel has its advantage that can be directly applied in a breeding program. In this study, we conducted a genome-wide association study (GWAS) for blast resistance using 296 commercial rice cultivars with low population structure but large phenotypic diversity. We attempt to answer the genetic basis behind rice blast resistance among early maturing cultivars by subdividing the population based on its () functionality. Subpopulation-specific GWAS using the mixed linear model (MLM) based on blast nursery screening conducted in three years revealed a total of 26 significant signals, including three nucleotide-binding site leucine-rich repeat (NBS-LRR) genes (, , and ) located at locus on chromosome 6, and one at the locus () on chromosome 12. Haplotype analysis revealed blast resistance associated with locus was exclusively specific to Type 14 among rice. Our findings provide valuable insights for breeding blast resistant rice and highlight the applicability of our elite cultivar panel to detect superior alleles associated with important agronomic traits.
稻瘟病是一种具有破坏性的真菌病害，会在水稻生长的各个阶段影响植株，严重威胁全球水稻产量的稳定性。培育抗病水稻品种是一种切实可行的病害防治方法。一般来说，利用多样性群体进行关联分析能够有效地鉴定出控制目标性状的新等位基因。另一方面，育种群体的利用具有可直接应用于育种计划的优势。在本研究中，我们使用296个群体结构简单但表型多样性丰富的商业水稻品种进行了全基因组关联研究（GWAS）以分析稻瘟病抗性。我们试图通过根据其（）功能对群体进行细分来回答早熟品种中稻瘟病抗性背后的遗传基础。基于三年稻瘟病圃筛选，使用混合线性模型（MLM）进行亚群特异性GWAS分析，共鉴定出26个显著信号，包括位于第6号染色体上 位点的三个核苷酸结合位点富含亮氨酸重复序列（NBS-LRR）基因（、 和 ），以及位于第12号染色体上 位点（）的一个基因。单倍型分析表明，与 位点相关的稻瘟病抗性在 水稻中仅特定于第14类型。我们的研究结果为培育抗稻瘟病水稻提供了有价值的见解，并突出了我们的优良品种群体在检测与重要农艺性状相关的优异等位基因方面的适用性。
