Li Chenggang, Wang Dan, Peng Shasha, Chen Yue, Su Pin, Chen Jianbin, Zheng Limin, Tan Xinqiu, Liu Jinling, Xiao Yinghui, Kang Houxiang, Zhang Deyong, Wang Guo-Liang, Liu Yong
State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, 410125, China.
Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China and College of Agronomy, Hunan Agricultural University, Changsha, 410128, Hunan, China.
Rice (N Y). 2019 Jul 15;12(1):47. doi: 10.1186/s12284-019-0309-7.
Effective management of rice blast, caused by the fungus Magnaporthe oryzae, requires an understanding of the genetic architecture of the resistance to the disease in rice. Rice resistance varies with M. oryzae strains, and many quantitative trait loci (QTLs) affecting rice blast resistance have been mapped using different strains of M. oryzae from different areas. However, little is known about the genetic architecture of rice resistance against the M. oryzae population in Hunan Province, which is a main rice production area in South China.
In this study, we used three isolates from Hunan Province and the rice diversity panel 1 to perform a genome-wide association study (GWAS) of blast resistance in rice. A total of 56 QTLs were identified. One of the QTLs is localized with the resistance gene Pik locus which confers resistance to all three isolates. Genomic sequence analysis of the resistant cultivars led to the identification of a new Pik allele, which we named Pikx. Yeast two-hybrid and co-immunoprecipitation assays between AvrPiks and Pikx confirmed that Pikx is a new allele at the Pik locus.
Our GWAS has identified many new blast resistance QTLs. The identified new Pik allele Pikx will be useful for breeding cultivars with high resistance to blast in Hunan and other South China provinces. Further research on the relationship between AvrPiks and Pikx will provide new insights into the molecular mechanism of rice resistance to M. oryzae.
稻瘟病由稻瘟病菌（Magnaporthe oryzae）引起，对其进行有效管理需要了解水稻对该病抗性的遗传结构。水稻抗性因稻瘟病菌株而异，利用来自不同地区的不同稻瘟病菌株已定位了许多影响水稻抗稻瘟病的数量性状基因座（QTL）。然而，对于中国南方主要水稻产区湖南省的水稻对稻瘟病菌群体的抗性遗传结构知之甚少。
在本研究中，我们使用来自湖南省的三个分离株和水稻多样性面板1对水稻的抗稻瘟病进行全基因组关联研究（GWAS）。共鉴定出56个QTL。其中一个QTL定位于抗性基因Pik位点，该位点对所有三个分离株均具有抗性。对抗性品种的基因组序列分析导致鉴定出一个新的Pik等位基因，我们将其命名为Pikx。AvrPiks与Pikx之间的酵母双杂交和免疫共沉淀试验证实Pikx是Pik位点的一个新等位基因。
我们的GWAS鉴定出许多新的抗稻瘟病QTL。鉴定出的新Pik等位基因Pikx将有助于在湖南和中国南方其他省份培育对稻瘟病具有高抗性的品种。对AvrPiks与Pikx之间关系的进一步研究将为水稻对稻瘟病菌抗性的分子机制提供新的见解。
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