Ma Jian, Lei Cailin, Xu Xingtao, Hao Kun, Wang Jiulin, Cheng Zhijun, Ma Xiaoding, Ma Jin, Zhou Kunneng, Zhang Xin, Guo Xiuping, Wu Fuqing, Lin Qibing, Wang Chunming, Zhai Huqu, Wang Haiyang, Wan Jianmin
1Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China;
2National Key Laboratory of Crop Genetics and Germplasm Enhancement / Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China.
Mol Plant Microbe Interact. 2015 May;28(5):558-68. doi: 10.1094/MPMI-11-14-0367-R.
Rice blast caused by Magnaporthe oryzae poses a major threat to rice production worldwide. The utilization of host resistance (R) genes is considered to be the most effective and economic means to control rice blast. Here, we show that the japonica landrace Yangmaogu (YMG) displays a broader spectrum of resistance to blast isolates than other previously reported broad-spectrum resistant (BSR) cultivars. Genetic analysis suggested that YMG contains at least three major R genes. One gene, Pi64, which exhibits resistance to indica-sourced isolate CH43 and several other isolates, was mapped to a 43-kb interval on chromosome 1 of YMG. Two open reading frames (NBS-1 and NBS-2) encoding nucleotide-binding site and leucine-rich repeat proteins were short-listed as candidate genes for Pi64. Constructs containing each candidate gene were transformed into three susceptible japonica cultivars. Only transformants with NBS-2 conferred resistance to leaf and neck blast, validating the idea that NBS-2 represents the functional Pi64 gene. Pi64 is constitutively expressed at all development stages and in all tissues examined. Pi64 protein is localized in both the cytoplasm and nucleus. Furthermore, introgression of Pi64 into susceptible cultivars via gene transformation and marker-assisted selection conferred high-level and broad-spectrum leaf and neck blast resistance to indica-sourced isolates, demonstrating its potential utility in breeding BSR rice cultivars.
由稻瘟病菌（Magnaporthe oryzae）引起的稻瘟病对全球水稻生产构成了重大威胁。利用寄主抗性（R）基因被认为是控制稻瘟病最有效且经济的手段。在此，我们表明粳稻地方品种阳冒谷（YMG）对稻瘟病菌株的抗性谱比其他先前报道的广谱抗性（BSR）品种更广。遗传分析表明YMG至少含有三个主要的R基因。其中一个基因Pi64，对来自籼稻的分离株CH43及其他几个分离株表现出抗性，被定位到YMG第1染色体上一个43 kb的区间内。两个编码核苷酸结合位点和富含亮氨酸重复序列蛋白的开放阅读框（NBS - 1和NBS - 2）被列为Pi64的候选基因。将包含每个候选基因的构建体转化到三个感病粳稻品种中。只有携带NBS - 2的转化体对叶瘟和穗颈瘟具有抗性，证实了NBS - 2代表功能性Pi64基因的观点。Pi64在所有发育阶段和所有检测的组织中都组成型表达。Pi64蛋白定位于细胞质和细胞核中。此外，通过基因转化和标记辅助选择将Pi64导入感病品种，赋予了对来自籼稻的分离株高水平和广谱的叶瘟和穗颈瘟抗性，证明了其在培育BSR水稻品种中的潜在应用价值。
