Department of Orthodontics, Khanzad Teaching Center, Ministry of Health, Erbil, Kurdistan Region- Iraq.
Department of Pedodontics, Orthodontics and Preventive Dentistry, Collage of Dentistry, Hawler Medical University, Erbil, Kurdistan Region- Iraq.
Cell Mol Biol (Noisy-le-grand). 2024 Jun 5;70(6):217-223. doi: 10.14715/cmb/2024.70.6.33.
Mesenchymal stem cells from bone marrow, such as bone marrow aspirate concentrate (BMAC) and cultured and isolated bone marrow mesenchymal stem cells (BM-MSCs), have been used as therapeutic alternatives to enhance remodeling in the bone.
This study aimed to evaluate the effects of BMAC and BM-MSCs on orthodontic tooth movements in rabbits.
A100- gram nickel-titanium closed-coil springs were used to initiate orthodontic tooth movement of the lower first premolars in 35 male New Zealand rabbits for 21 days. Using a split-mouth design, autologous BMAC or BM-MSCs were submucosally injected into the right sides of the lower jaw, while the left sides served as the control. On days 7, 14, and 21, a three-dimensional digital model scan was used to measure the amount of tooth movement. The microfocus computed tomography (Micro-CT) and histological findings were examined on day 0 as the baseline measurement and on days 7, 14, and 21.
Compared to the control group, the quadrant receiving BMAC and BM-MSCs had a considerably greater amount of tooth movement. Histomorphometric analysis revealed that both BMAC and BM-MSCs had significantly higher numbers of osteoclasts and active bone-resorptive lacunae. The resorptive changes were greater in the BMAC and BM-MSCs groups than in the control group.
The submucosal injection of BMAC and BM-MSCs accelerates orthodontic tooth movement (OTM) by decreasing bone density and supplying more osteoclast progenitor cells.
骨髓间充质干细胞，如骨髓抽吸浓缩物（BMAC）和培养分离的骨髓间充质干细胞（BM-MSCs），已被用作增强骨骼重塑的治疗替代物。
本研究旨在评估 BMAC 和 BM-MSCs 对兔正畸牙齿移动的影响。
使用 100 克镍钛封闭线圈弹簧启动下第一前磨牙的正畸牙齿移动，持续 21 天。使用分侧设计，将自体 BMAC 或 BM-MSCs 黏膜下注射到下颌右侧，而左侧作为对照。在第 7、14 和 21 天，使用三维数字模型扫描测量牙齿移动量。在第 0 天（即基线测量）和第 7、14 和 21 天进行微焦点计算机断层扫描（Micro-CT）和组织学检查。
与对照组相比，接受 BMAC 和 BM-MSCs 的象限牙齿移动量明显更大。组织形态计量学分析显示，BMAC 和 BM-MSCs 组的破骨细胞和活跃的骨吸收陷窝数量明显更高。在 BMAC 和 BM-MSCs 组中，吸收变化大于对照组。
BMAC 和 BM-MSCs 的黏膜下注射通过降低骨密度并提供更多的破骨细胞祖细胞来加速正畸牙齿移动（OTM）。
