Kurdistan Regional Government | Ministry of Health, General directorate of health, Erbil 44001 Iraq.
Department of Conservative Dentistry, College of Dentistry, Hawler Medical University, Erbil 44001, Iraq.
Cell Mol Biol (Noisy-le-grand). 2024 Jun 5;70(6):135-141. doi: 10.14715/cmb/2024.70.6.21.
Epigenetic change has been found to play an important role in cell differentiation and regulation and the dental pulp stem cell in tissue engineering is gaining attention due to the ability of cells to differentiate into odontoblast and other cells. This study evaluated the influence of poly L- lactic acid with hydroxyapatite-coated with polyaniline scaffold (PLLA/HA/PANI) on dental pulp stem cell (DPSC) proliferation and differentiation. After scaffold preparation and DPSCs seeding, the cells proliferation and differentiation were evaluated by immunocytochemistry assay and cell viability was measured by cytotoxicity / MTT assay. The results showed (PLLA/HA/PANI) scaffold facilitates DPSC proliferation and differentiation with gene expression. This finding underscores the promise of this biomaterial combination as a scaffold for dental tissue regeneration and application.
表观遗传改变被发现对细胞分化和调控起着重要作用，牙髓干细胞在组织工程中受到关注，因为其具有分化为成牙本质细胞和其他细胞的能力。本研究评估了聚左旋乳酸与羟基磷灰石/聚苯胺涂层支架（PLLA/HA/PANI）对牙髓干细胞（DPSC）增殖和分化的影响。在支架制备和 DPSCs 接种后，通过免疫细胞化学检测评估细胞增殖和分化，通过细胞毒性/MTT 检测评估细胞活力。结果表明，（PLLA/HA/PANI）支架促进了 DPSC 的增殖和基因表达诱导的分化。这一发现强调了这种生物材料组合作为牙齿组织再生和应用支架的潜力。
