Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Pharm Nanotechnol. 2024;12(3):262-268. doi: 10.2174/2211738511666230817102159.
In recent years, the electrospinning method has received attention because of its usage in producing a mimetic nanocomposite scaffold for tissue regeneration. Hydroxyapatite and gelatin are suitable materials for producing scaffolds, and curcumin has the osteogenesis induction effect.
This study aimed to evaluate the toxicity and early osteogenic differentiation stimulation of nanofibrous gelatin-hydroxyapatite scaffold containing curcumin on dental pulp stem cells (DPSCs).
The objective of the present investigation was the evaluation of the proliferative effect and primary osteogenic stimulation of DPSCs with a nanofibrous gelatin-hydroxyapatite scaffold containing curcumin. Hydroxyapatite and gelatin were used as suitable and biocompatible materials to make a scaffold suitable for stimulating osteogenesis. Curcumin was added to the scaffold as an osteogenic differentiation- enhancing agent.
The effect of nano-scaffold on the proliferation of DPSCs was evaluated. The activity of alkaline phosphatase (ALP) as the early osteogenic marker was considered to assess primary osteogenesis stimulation in DPSCs.
The nanofibrous gelatin-hydroxyapatite scaffold containing curcumin significantly increased the proliferation and the ALP activity of DPSCs (P<0.05). The proliferative effect was insignificant in the first 2 days, but the scaffold increased cell proliferation by more than 40% in the fourth and sixth days. The prepared scaffold increased the activity of the ALP of DPSCs by 60% compared with the control after 14 days (p<0.05).
The produced nanofibrous gelatin-hydroxyapatite scaffold containing curcumin can be utilized as a potential candidate in tissue engineering and regeneration of bone and tooth.
The prepared scaffold in the present study could be a beneficial biomaterial for tissue engineering and the regeneration of bone and tooth soon.
近年来，电纺方法因其在组织再生仿生纳米复合支架制备中的应用而受到关注。羟基磷灰石和明胶是适合制备支架的材料，姜黄素具有成骨诱导作用。
本研究旨在评价含姜黄素的纳米纤维明胶-羟基磷灰石支架对牙髓干细胞（DPSCs）的毒性和早期成骨分化刺激作用。
本研究旨在评价含姜黄素的纳米纤维明胶-羟基磷灰石支架对牙髓干细胞（DPSCs）的增殖效应和初步成骨刺激作用。羟基磷灰石和明胶被用作合适和生物相容的材料来制备适合刺激成骨的支架。姜黄素被添加到支架中作为成骨分化增强剂。
评价纳米支架对 DPSCs 增殖的影响。考虑碱性磷酸酶（ALP）作为早期成骨标志物的活性，以评估 DPSCs 中的初步成骨刺激作用。
含姜黄素的纳米纤维明胶-羟基磷灰石支架显著增加了 DPSCs 的增殖和 ALP 活性（P<0.05）。在前 2 天，增殖效应不显著，但支架在第 4 天和第 6 天使细胞增殖增加了 40%以上。与对照组相比，在第 14 天，制备的支架使 DPSCs 的 ALP 活性增加了 60%（p<0.05）。
含姜黄素的纳米纤维明胶-羟基磷灰石支架可作为组织工程和骨牙再生的潜在候选材料。
本研究中制备的支架很快将成为组织工程和骨牙再生的有益生物材料。
