Xia Ji'an, Zhang Yu, Zhang Suhua, Lu Chunlai, Huan Hongdi, Guan Xiao
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China.
National Grain Industry (Urban Grain and Oil Security) Technology Innovation Center, Shanghai 200093, China.
J Agric Food Chem. 2024 Jun 5. doi: 10.1021/acs.jafc.4c02591.
Chronic kidney disease (CKD) has emerged as a significant public health concern. In this article, we investigated the mechanism of oat dietary fiber in regulating CKD. Our findings indicated that the gut microbiota of CKD patients promoted gut microbiota dysbiosis and kidney injury in CKD mice. Intervention with oat-resistant starch prepared by ultrasonic combined enzymatic hydrolysis (ORSU) and oat β-glucan with a molecular weight of 5 × 10 Da (OBGM) elevated the levels of short-chain fatty acids (SCFAs) and regulated gut dysbiosis in the gut-humanized CKD mice. ORSU and OBGM also reduced CKD-related uremic toxins such as creatinine, indoxyl sulfate (IS), and -cresol sulfate (PCS) levels; reinforced the intestinal barrier function of the gut-humanized CKD mice; and mitigated renal inflammation and fibrosis via the NF-κB/TGF-β pathway. Therefore, ORSU and OBGM might delay the progression of CKD by modulating the gut microbiota to reduce uremic toxins levels. Our results explain the mechanism of oat dietary fiber aimed at mitigating CKD.
慢性肾脏病（CKD）已成为一个重大的公共卫生问题。在本文中，我们研究了燕麦膳食纤维调节CKD的机制。我们的研究结果表明，CKD患者的肠道微生物群促进了CKD小鼠的肠道微生物群失调和肾脏损伤。用超声联合酶解法制备的燕麦抗性淀粉（ORSU）和分子量为5×10 Da的燕麦β-葡聚糖（OBGM）进行干预，可提高肠道人源化CKD小鼠的短链脂肪酸（SCFAs）水平并调节肠道失调。ORSU和OBGM还降低了与CKD相关的尿毒症毒素，如肌酐、硫酸吲哚酚（IS）和对甲酚硫酸盐（PCS）的水平；增强了肠道人源化CKD小鼠的肠道屏障功能；并通过NF-κB/TGF-β途径减轻了肾脏炎症和纤维化。因此，ORSU和OBGM可能通过调节肠道微生物群以降低尿毒症毒素水平来延缓CKD的进展。我们的结果解释了燕麦膳食纤维减轻CKD的机制。
