Ikeda T, Mokuda O, Kuno S, Tokumori Y, Tominaga M, Mashiba H
Am J Physiol. 1985 Mar;248(3 Pt 1):E304-8. doi: 10.1152/ajpendo.1985.248.3.E304.
To study the intestinal insulinotropic effects in the diabetic state, an investigation was made into the release of insulin from isolated rat pancreas perfused with portal venous effluent (PVE) obtained from the isolated perfused intestine of streptozotocin-induced diabetic rats. Rat intestine was perfused with Krebs-Ringer bicarbonate medium for 1 h, and the PVE was collected from both untreated and glucose-treated intestines of control and diabetic rats. The PVE, after adjusting its glucose concentration to the desired level, was used as the perfusing medium for the pancreas of a different rat. Glucose concentration in the perfusion medium was maintained at 5.5 mM for 20 min and at 16.7 mM for the next 30 min. Insulin output from pancreas perfused with PVE from untreated intestine of diabetic rats (252 +/- 45 ng/30 min, mean +/- SD) was similar to that of controls (269 +/- 72 ng/30 min). Pancreatic insulin output with PVE from glucose-treated intestine of diabetic rats (579 +/- 100 ng/30 min) was significantly greater compared with either that using PVE from untreated intestine of diabetic rats (P less than 0.01) or that from glucose-treated intestine of control rats (368 +/- 57 ng/30 min) (P less than 0.02). These results indicate that the insulinotropic effect is markedly enhanced in streptozotocin-induced diabetic rats.
为研究糖尿病状态下的肠道促胰岛素作用，对用链脲佐菌素诱导的糖尿病大鼠离体灌注肠所得的门静脉流出液（PVE）灌注的离体大鼠胰腺释放胰岛素的情况进行了研究。用Krebs-Ringer碳酸氢盐培养基对大鼠肠道灌注1小时，从对照大鼠和糖尿病大鼠未经处理及葡萄糖处理的肠道收集PVE。将PVE的葡萄糖浓度调整至所需水平后，用作另一只大鼠胰腺的灌注培养基。灌注培养基中的葡萄糖浓度在20分钟内维持在5.5 mM，在接下来的30分钟内维持在16.7 mM。用糖尿病大鼠未经处理肠道的PVE灌注的胰腺胰岛素输出量（252±45 ng/30分钟，均值±标准差）与对照组（269±72 ng/30分钟）相似。糖尿病大鼠经葡萄糖处理肠道的PVE灌注的胰腺胰岛素输出量（579±100 ng/30分钟），与使用糖尿病大鼠未经处理肠道的PVE（P<0.01）或对照大鼠经葡萄糖处理肠道的PVE（368±57 ng/30分钟）相比（P<0.02），显著更高。这些结果表明，在链脲佐菌素诱导的糖尿病大鼠中，促胰岛素作用明显增强。
