Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan.
J Hepatobiliary Pancreat Sci. 2024 Sep;31(9):611-624. doi: 10.1002/jhbp.12012. Epub 2024 Jun 4.
Liver metastasis is the most frequently observed distant metastasis of colorectal cancer, and the residual liver recurrence rate after hepatic resection is still high. To explore the mechanism of liver metastasis to discover potential new treatments, we assessed the relationship between the expression of differentially expressed genes (DEGs) and prognosis in patients with colorectal cancer liver metastasis (CRLM).
The gene expression dataset was extracted from The Cancer Genome Atlas and the Gene Expression Omnibus. Significance analysis of DEGs between tumor and normal samples of colorectum, liver, and lung was conducted. A total of 80 CRLM patients were studied to assess the expression of RPS15, characteristics, and outcomes. We examined the relationships of RPS15 expression to cell viability and apoptosis in vitro and vivo.
Significance analysis identified 33 DEGs. In our cohorts, the overall survival rates were significantly lower in the high-RPS15-expression group, and high expression of RPS15 was an independent and unfavorable prognostic factor in recurrence-free survival and overall survival. Knockdown of RPS15 expression reduced the proliferative capacity of colorectal cancer cells and increased BAX-induced apoptotic cell death.
RPS15 expression is an independent prognostic factor for CRLM patients and might be a novel therapeutic target for CRLM.
肝转移是结直肠癌最常见的远处转移，肝切除术后残留肝脏复发率仍较高。为了探讨肝转移的机制，寻找潜在的新治疗方法，我们评估了结直肠癌肝转移（CRLM）患者差异表达基因（DEGs）表达与预后的关系。
从癌症基因组图谱和基因表达综合数据库中提取基因表达数据集。对结直肠、肝和肺的肿瘤与正常样本之间的 DEGs 进行显著性分析。对 80 例 CRLM 患者进行研究，评估 RPS15 的表达、特征和结果。我们在体外和体内研究了 RPS15 表达与细胞活力和细胞凋亡的关系。
显著性分析鉴定出 33 个 DEGs。在我们的队列中，高 RPS15 表达组的总生存率显著降低，RPS15 高表达是无复发生存和总生存的独立不良预后因素。RPS15 表达的敲低降低了结直肠癌细胞的增殖能力，并增加了 BAX 诱导的凋亡细胞死亡。
RPS15 表达是 CRLM 患者的独立预后因素，可能是 CRLM 的一个新的治疗靶点。
