Tremoli E, Jaffe E A, Goldman K T, Weksler B B
Arteriosclerosis. 1985 Mar-Apr;5(2):178-85. doi: 10.1161/01.atv.5.2.178.
The influence of hyperlipidemic sera on prostacyclin (PGI2) production by cultured endothelial cells was assessed by comparing sera from three types of hyperlipidemias with sera from normal subjects. Sera prepared from normal whole blood (WBS), platelet-rich plasma (PRPS), and platelet-poor plasma (PPPS) were also compared. Bovine aortic endothelial cells (BAEC) incubated with 25% WBS increased PGI2 synthesis significantly within 1 hour, with little further increase by 16 hours; human umbilical vein endothelial cells (HUEC) incubated with 25% WBS for 1 hour showed no elevation in PGI2, whereas PGI2 levels increased substantially after 16 hours. PPPS and PRPS stimulated PGI2 synthesis by BAEC equally at 1 hour. However, there was no rise in PGI2 after PPPS in HUEC; PGI2 rose after 16 hours with PRPS and rose further with WBS after 16 hours. Since WBS best enhanced PGI2 production in human endothelial cells, it was chosen for comparison of the effects of hyperlipidemic and normolipidemic sera. PGI2 synthesis by HUEC significantly increased upon incubation with WBS from Types IIb and IV patients in comparison to WBS from Type IIa hypercholesterolemic patients or normal controls. In contrast, WBS from all these hyperlipidemic subjects stimulated PGI2 synthesis by BAEC similarly to WBS from controls. We conclude that incubation of human endothelial cells with WBS containing high levels of atherogenic lipoproteins does not reduce PGI2 formation by the cells. Moreover, the time course and the contribution of lipid, plasma, or cellular factors to PGI2 formation vary according to the cell type tested. Caution should be exercised in extrapolating results achieved with serum and cells from the same species to other settings.
通过比较三种高脂血症患者的血清与正常受试者的血清，评估了高脂血症血清对培养的内皮细胞产生前列环素（PGI2）的影响。还比较了从正常全血（WBS）、富血小板血浆（PRPS）和贫血小板血浆（PPPS）制备的血清。用25% WBS孵育的牛主动脉内皮细胞（BAEC）在1小时内PGI2合成显著增加，到16小时时进一步增加很少；用25% WBS孵育1小时的人脐静脉内皮细胞（HUEC）PGI2没有升高，而16小时后PGI2水平大幅升高。PPPS和PRPS在1小时时同样刺激BAEC的PGI2合成。然而，HUEC中PPPS处理后PGI2没有升高；PRPS处理16小时后PGI2升高，WBS处理16小时后PGI2进一步升高。由于WBS能最佳地增强人内皮细胞中PGI2的产生，因此选择它来比较高脂血症和正常血脂血清的作用。与IIa型高胆固醇血症患者或正常对照的WBS相比，用IIb型和IV型患者的WBS孵育时，HUEC的PGI2合成显著增加。相比之下，所有这些高脂血症受试者的WBS刺激BAEC的PGI2合成与对照的WBS相似。我们得出结论，用人内皮细胞与含有高水平致动脉粥样硬化脂蛋白的WBS孵育不会降低细胞的PGI2形成。此外，脂质、血浆或细胞因子对PGI2形成的时间进程和贡献因所测试的细胞类型而异。在将同一物种的血清和细胞所获得的结果外推到其他情况时应谨慎。
