Ojaghi Habib, Poorsheykhian Shirin, Najafi Amin, Iranpour Sohrab
Department of Surgery, Ardabil University of Medical Sciences, Ardabil, Iran.
MD, Ardabil University of Medical Sciences, Ardabil, Iran.
Immun Ageing. 2024 Jun 5;21(1):35. doi: 10.1186/s12979-024-00440-5.
Age-related macular degeneration (AMD) is a significant retinal disease that leads to irreversible low vision, particularly in developing countries. The variation in AMD prevalence among different racial groups and highlighted role of inflammation on disease pathology from previous studies which yielded in inconsistent findings, It seems to be of great importance to do more investigation in this field.
This case control study involved 204 participants, divided into four groups of equal size (51 individuals per group). Three groups represented AMD cases of varying severity according to Beckman classification (3 groups) and one healthy control group. Sampling was conducted exhaustively until the desired sample size was reached. The control group comprised healthy individuals without any infectious or inflammatory systemic, ophthalmic disease. Blood samples were collected to measure inflammatory factors, including lymphocytes, monocytes, neutrophils, neutrophil-to-lymphocyte ratio (NLR), and C-reactive protein (CRP). Collected data were analyzed by statistical methods in SPSS version 21.
Of the participants, 51% were women, and their ages ranged from 47 to 89 years (62.2 ± 8). According to multiple logistic regression analysis, age exhibited a statistically significant positive association with AMD severity (P = 0.038, odds ratio [OR] = 1.034). ANOVA results indicated a significant association between neutrophil count and AMD severity (P < 0.001). As the disease severity increased, the number of neutrophils decreased. The mean ± SD neutrophil counts for early, intermediate and advanced AMD were 3849 ± 800, 3702 ± 734, and 3342 ± 823, respectively. No statistically significant associations were found between lymphocyte count, monocyte count, neutrophil-to-lymphocyte ratio, CRP, and AMD.
There was a significant relationship between the number of neutrophils in peripheral blood and the severity of AMD in study participants which needs more evaluation for the potential utility of this factor in the prognosis of AMD. There was not any significant relationship among the other factors and AMD.
年龄相关性黄斑变性（AMD）是一种严重的视网膜疾病，会导致不可逆的视力低下，在发展中国家尤为如此。先前研究中不同种族群体间AMD患病率的差异以及炎症在疾病病理过程中的突出作用，但结果并不一致，因此在该领域开展更多研究似乎非常重要。
本病例对照研究纳入了204名参与者，平均分为四组（每组51人）。三组根据贝克曼分类法代表不同严重程度的AMD病例（3组），一组为健康对照组。进行全面抽样，直至达到所需样本量。对照组由无任何感染性或炎症性全身性、眼科疾病的健康个体组成。采集血样以测量炎症因子，包括淋巴细胞、单核细胞、中性粒细胞、中性粒细胞与淋巴细胞比值（NLR）和C反应蛋白（CRP）。收集的数据用SPSS 21版统计方法进行分析。
参与者中，51%为女性，年龄范围为47至89岁（62.2±8）。根据多因素逻辑回归分析，年龄与AMD严重程度呈统计学显著正相关（P = 0.038，比值比[OR]=1.034）。方差分析结果表明中性粒细胞计数与AMD严重程度之间存在显著关联（P<0.001）。随着疾病严重程度增加，中性粒细胞数量减少。早期、中期和晚期AMD的中性粒细胞平均计数±标准差分别为3849±800、3702±734和3342±823。淋巴细胞计数、单核细胞计数、中性粒细胞与淋巴细胞比值、CRP与AMD之间未发现统计学显著关联。
研究参与者外周血中性粒细胞数量与AMD严重程度之间存在显著关系，该因素在AMD预后中的潜在效用需要更多评估。其他因素与AMD之间无显著关系。
