Ma Hong, Steede Tyler, Dewey Ralph E, Lewis Ramsey S
Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina 27695, United States.
J Agric Food Chem. 2024 Jun 5. doi: 10.1021/acs.jafc.4c02442.
Sclareol, a diterpene alcohol, is the most common starting material for the synthesis of ambrox, which serves as a sustainable substitute for ambergris, a valuable fragrance secreted by sperm whales. Sclareol has also been proposed to possess antibacterial, antifungal, and anticancer activities. However, in nature, sclareol is only produced by a few plant species, including , , , and , which limits its commercial application. In this study, we cloned the two genes responsible for sclareol biosynthesis in , labda-13-en-8-ol diphosphate synthase () and sclareol synthase (), and overexpressed them in tobacco ( L.). The best transgenic tobacco lines accumulated 4.1 μg/cm of sclareol, which is comparable to the sclareol production of , a natural sclareol producer. Thus, sclareol synthesis in tobacco represents a potential alternative means for the production of this high-value compound.
香紫苏醇是一种二萜醇，是合成龙涎醚的最常见起始原料，龙涎醚可作为抹香鲸分泌的一种珍贵香料龙涎香的可持续替代品。香紫苏醇也被认为具有抗菌、抗真菌和抗癌活性。然而，在自然界中，香紫苏醇仅由少数几种植物产生，包括[此处原文缺失植物名称]，这限制了其商业应用。在本研究中，我们克隆了[此处原文缺失植物名称]中负责香紫苏醇生物合成的两个基因，13- labda -烯-8-醇二磷酸合酶（[此处原文缺失基因名称]）和香紫苏醇合酶（[此处原文缺失基因名称]），并在烟草（[此处原文缺失烟草品种名称]）中过表达它们。最佳转基因烟草品系积累了4.1μg/cm的香紫苏醇，这与天然香紫苏醇生产者[此处原文缺失植物名称]的香紫苏醇产量相当。因此，烟草中的香紫苏醇合成代表了生产这种高价值化合物的一种潜在替代方法。
