Li Zhen, Zhong Shiqing, Huang Qiang, Zhang Yong, Xu Tianyu, Shi Wenkai, Guo Dongsheng, Zeng Zhijiang
Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China.
Jiangxi Province Key Laboratory of Honeybee Biology and Beekeeping, Nanchang 330045, China.
iScience. 2024 Apr 29;27(6):109847. doi: 10.1016/j.isci.2024.109847. eCollection 2024 Jun 21.
is an economically and medicinally valuable oilseed crop. Honeybee, the most abundant pollinator, rarely visits because of the toxic sugars in the nectar and pollen. These toxic sugars cannot be fully digested by honeybees and inhibit the process of synthesizing trehalose in honeybees. exhibits self-incompatibility, and its pollination heavily depends on . However, the mechanism by which digests toxic sugars in nectar and pollen remains unknown. Consequently, we identified and validated four single-copy genes (, , , and , abbreviated as , , , and ) essential for detoxifying toxic sugars . Then, we cloned the four genes into , and expressed enzyme successfully degraded the toxic sugars. The phylogeny suggests that the genes were conserved and functionally diverged among the evolution. These results provide novel insights into pollinator detoxification during co-evolution.
是一种具有经济和药用价值的油料作物。蜜蜂是最丰富的传粉者，但由于花蜜和花粉中的有毒糖分，很少光顾。这些有毒糖分不能被蜜蜂完全消化，并抑制蜜蜂体内海藻糖的合成过程。表现出自交不亲和性，其授粉严重依赖于。然而，消化花蜜和花粉中有毒糖分的机制仍然未知。因此，我们鉴定并验证了四个对解毒有毒糖分至关重要的单拷贝基因（、、、，简称为、、、）。然后，我们将这四个基因克隆到中，表达的酶成功降解了有毒糖分。系统发育表明，这些基因在进化过程中是保守的且功能发生了分化。这些结果为共同进化过程中传粉者解毒提供了新的见解。
