Shengli Clinical Medical College of Fujian Medical University, Fujian, Fuzhou, China.
Department of Radiology, Fujian Provincial Hospital, Fujian, Fuzhou, China.
Front Immunol. 2024 May 22;15:1391280. doi: 10.3389/fimmu.2024.1391280. eCollection 2024.
Currently, there is a lack of an objective quantitative measure to comprehensively evaluate the inflammatory activity of axSpA, which poses certain challenges in accurately assessing the disease activity.
To explore the value of combined-parameter models of sacroiliac joints (SIJs) MRI relaxometry and peripheral blood Mucosal-associated invariant T (MAIT) cells in evaluating the inflammatory activity of axial spondyloarthritis (axSpA).
This retrospective clinical study included 88 axSpA patients (median age 31.0 (22.0, 41.8) years, 21.6% females) and 20 controls (median age 28.0 (20.5, 49.5) years, 40.0% females). The axSpA group was classified into active subgroup (n=50) and inactive subgroup (n=38) based on ASDAS-CRP. All participants underwent SIJs MRI examination including T1 and T2* mapping, and peripheral blood flow cytometry analysis of MAIT cells (defined as CD3Vα7.2CD161) and their activation markers (CD69). The T1 and T2* values, as were the percentages of MAIT cells and CD69MAIT cells were compared between different groups. Combined-parameter models were established using logistic regression, and ROC curves were employed to evaluate the diagnostic efficacy.
The T1 values of SIJs and %CD69MAIT cells in the axSpA group and its subgroup were higher than the control group (p<0.05), while %MAIT cells were lower than the control group (p<0.05). The T1 values and %CD69MAIT cells correlated positively, while %MAIT cells correlated negatively, with the ASDAS-CRP (r=0.555, 0.524, -0.357, p<0.001). Between the control and axSpA groups, and between the inactive and active subgroups, the combined-parameter model T1 mapping+%CD69MAIT cells has the best efficacy (AUC=0.959, 0.879, sensibility=88.6, 70%, specificity=95.0, 94.7%, respectively).
The combined-parameter model T1 mapping+%CD69MAIT cells allows a more accurate evaluation of the level of inflammatory activity.
目前，缺乏一种客观的定量方法来全面评估 axSpA 的炎症活动，这给准确评估疾病活动带来了一定的挑战。
探讨骶髂关节（SIJ）MRI 弛豫度和外周血黏膜相关不变 T（MAIT）细胞联合参数模型在评估轴性脊柱关节炎（axSpA）炎症活动中的价值。
本回顾性临床研究纳入了 88 例 axSpA 患者（中位年龄 31.0（22.0，41.8）岁，21.6%为女性）和 20 名对照者（中位年龄 28.0（20.5，49.5）岁，40.0%为女性）。根据 ASDAS-CRP，将 axSpA 组分为活动亚组（n=50）和非活动亚组（n=38）。所有参与者均接受 SIJ MRI 检查，包括 T1 和 T2* 成像，以及外周血 MAIT 细胞（定义为 CD3Vα7.2CD161）及其激活标志物（CD69）的流式细胞术分析。比较不同组间 SIJ 的 T1 和 T2* 值以及 MAIT 细胞和 CD69MAIT 细胞的百分比。使用逻辑回归建立联合参数模型，并采用 ROC 曲线评估诊断效能。
axSpA 组及其亚组的 SIJ T1 值和%CD69MAIT 细胞高于对照组（p<0.05），而 MAIT 细胞百分比低于对照组（p<0.05）。T1 值和%CD69MAIT 细胞与 ASDAS-CRP 呈正相关（r=0.555，0.524，p<0.001），而 MAIT 细胞与 ASDAS-CRP 呈负相关（r=-0.357，p<0.001）。在对照组与 axSpA 组之间，以及在非活动亚组与活动亚组之间，T1 映射+%CD69MAIT 细胞联合参数模型的效能最佳（AUC=0.959，0.879，敏感性=88.6%，特异性=95.0%；AUC=0.879，0.700，敏感性=70%，特异性=94.7%）。
T1 映射+%CD69MAIT 细胞联合参数模型可更准确地评估炎症活动水平。
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